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Type: Artigo de periódico
Title: Simultaneous Quantification Of Phenolic Compounds In Buriti Fruit (mauritia Flexuosa L.f.) By Ultra-high Performance Liquid Chromatography Coupled To Tandem Mass Spectrometry
Author: Bataglion G.A.
da Silva F.M.A.
Eberlin M.N.
Koolen H.H.F.
Abstract: A novel analytical approach based on UHPLC-ESI(-)-MS/MS has been performed and evaluated for the identification and quantification of phenolic compounds (phenolic acids and flavonoids/catechins) in buriti (Mauritia flexuosa L.f.) fruit pulp. Due to the selectivity provided by MMR monitoring, UHPLC separation required only 10min per analysis. Linearity (0.994<r2<0.999), limit of detection (8.39-109.80pgmL-1) and limit of quantification (25.42-332.90pgmL-1), inter- and intraday accuracies (>80%) and precisions (CV<20%), and recovery rate (<80%) show that the developed method is reliable to analyze phenolic compounds in fruit extracts. Among the thirteen phenolic compounds analyzed by proposed method, buriti fruit pulp extract showed 6 phenolic acids such as p-coumaric acid, ferulic acid, caffeic acid, protocatechuic acid, chlorogenic acid and quinic acid and 7 flavonoids such as (+)-catechin, (-)-epicatechin, apigenin, luteolin, myricetin, kaempferol and quercetin. As a conclusion, the method was successfully developed and applied representing a contribution to the chemical characterization and quantification of phenolic compounds from buriti fruits which have acknowledged antioxidant and antimicrobial activities.
Editor: Elsevier Ltd
Rights: fechado
Identifier DOI: 10.1016/j.foodres.2014.09.035
Date Issue: 2014
Appears in Collections:Unicamp - Artigos e Outros Documentos

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