Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/85690
Type: Artigo de periódico
Title: Extraction Of Phenolic Compounds From Pitanga (eugenia Uniflora L.) Leaves By Sequential Extraction In Fixed Bed Extractor Using Supercritical Co 2, Ethanol And Water As Solvents
Author: Garmus T.T.
Paviani L.C.
Queiroga C.L.
Magalhaes P.M.
Cabral F.A.
Abstract: With the goal of maximizing the extraction yield of phenolic compounds from pitanga leaves (Eugenia uniflora L.), a sequential extraction in fixed bed was carried out in three steps at 60 C and 400 bar, using supercritical CO 2 (non-polar) as solvent in a first step, followed by ethanol (polarity: 5.2) and water (polarity: 9.0) in a second and third steps, respectively. All extracts were evaluated for global extraction yield, concentration and yield of both polyphenols and total flavonoids and antioxidant activity by DPPH method (in terms of EC50). The nature of the solvent significantly influenced the process, since the extraction yield increased with solvent polarity. The aqueous extracts presented higher global extraction yield (22%), followed by ethanolic (16%) and supercritical extracts (5%). The study pointed out that the sequential extraction process is the most effective in terms of global extraction yield and yield of polyphenols and total flavonoids, because it produced the more concentrated extracts on phenolic compounds, since the supercritical ethanolic extract presented the highest phenolics content (240.5 mg GAE/g extract) and antioxidant capacity (EC 50 = 9.15 μg/mL). The most volatile fraction from the supercritical extract, which is similar to the essential oils obtained by steam distillation or hydrodistillation, presented as major compounds the germacrenos D and B + bicyclogermacrene (40.75%), selina-1,3,7(11)-trien-8-one + selina-1,3,7(11)-trien-8-one epoxide (27.7%) and trans-caryophyllene (14.18%). © 2013 Elsevier B.V.
Editor: 
Rights: fechado
Identifier DOI: 10.1016/j.supflu.2013.11.014
Address: http://www.scopus.com/inward/record.url?eid=2-s2.0-84891111248&partnerID=40&md5=94e957629a159d20cc55b01b360962a7
Date Issue: 2014
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File Description SizeFormat 
2-s2.0-84891111248.pdf1.69 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.