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|Title:||The exocyst is required for trypanosome invasion and the repair of mechanical plasma membrane wounds|
|Author:||Fernandes, Maria Cecilia|
Miguel, Danilo C.
Andrews, Norma W.
|Abstract:||The process of host cell invasion by Trypanosoma cruzi shares mechanistic elements with plasma membrane injury and repair. Both processes require Ca2+-triggered exocytosis of lysosomes, exocytosis of acid sphingomyelinase and formation of ceramide-enriched endocytic compartments. T. cruzi invades at peripheral sites, suggesting a need for spatial regulation of membrane traffic. Here, we show that Exo70 and Sec8 (also known as EXOC7 and EXOC4, respectively), components of the exocyst complex, accumulate in nascent T. cruzi vacuoles and at sites of mechanical wounding. Exo70 or Sec8 depletion inhibits T. cruzi invasion and Ca2+-dependent resealing of mechanical wounds, but does not affect the repair of smaller lesions caused by pore-forming toxins. Thus, T. cruzi invasion and mechanical lesion repair share a unique requirement for the exocyst, consistent with a dependence on targetedmembrane delivery.|
The process of host cell invasion by Trypanosoma cruzi shares mechanistic elements with plasma membrane injury and repair. Both processes require Ca2+-triggered exocytosis of lysosomes, exocytosis of acid sphingomyelinase and formation of ceramide-enriche
|Subject:||Ferimentos e lesões|
|Editor:||The Company of Biologists|
|Citation:||Journal Of Cell Science. Company Of Biologists Ltd, v. 128, n. 1, p. 27 - 32, 2015.|
|Appears in Collections:||IB - Artigos e Outros Documentos|
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