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dc.typeArtigo de periódicopt_BR
dc.titleNanostructured Lipid Carriers As A Strategy To Improve The In Vitro Schistosomiasis Activity Of Praziquantelpt_BR
dc.contributor.authorKolenyak-Santos F.pt_BR
dc.contributor.authorGarnero C.pt_BR
dc.contributor.authorDe Oliveira R.N.pt_BR
dc.contributor.authorDe Souza A.L.R.pt_BR
dc.contributor.authorChorilli M.pt_BR
dc.contributor.authorAllegretti S.M.pt_BR
dc.contributor.authorLonghi M.R.pt_BR
dc.contributor.authorChaud M.V.pt_BR
dc.contributor.authorGremiao M.P.D.pt_BR
unicamp.authorDe Oliveira, R.N., Department of Biology Animal, Universidade Estadual de Campinas, UNICAMPSão Paulo, Brazilpt_BR
unicamp.authorAllegretti, S.M., Department of Biology Animal, Universidade Estadual de Campinas, UNICAMPSão Paulo, Brazilpt_BR, F., Program of Pharmaceutical Nanotechnology - UNESP-Araraquara, School of Pharmaceutical SciencesSão Paulo, Brazilpt, C., Facultad de Ciencias Químicas, Department of Pharmacy, Universidad Nacional de CórdobaCórdoba, Argentinapt Souza, A.L.R., Program of Pharmaceutical Science, School of Pharmaceutical Sciences, UNESPAraraquara, Brazilpt, M., Program of Pharmaceutical Science, School of Pharmaceutical Sciences, UNESPAraraquara, Brazilpt, M.R., Facultad de Ciencias Químicas, Department of Pharmacy, Universidad Nacional de CórdobaCórdoba, Argentinapt, M.V., Pharmaceutical Sciences Program, Sorocaba University, UNISOSorocaba, Brazilptão, M.P.D., Program of Pharmaceutical Nanotechnology - UNESP-Araraquara, School of Pharmaceutical SciencesSão Paulo, Brazil, Program of Pharmaceutical Science, School of Pharmaceutical Sciences, UNESPAraraquara, Brazilpt
dc.description.abstractPraziquantel (PZQ) is a pyrazinoisoquinoline anthelmintic that was discovered in 1972 by Bayer Germany. Currently, due to its efficacy, PZQ is the drug of choice against all species of Schistosoma. Although widely used, PZQ exhibits low and erratic bioavailability because of its poor water solubility. Nanostructured lipid carriers (NLC), second-generation solid lipid nanoparticles, were developed in the 1990s to improve the bioavailability of poorly water soluble drugs. The aim of this study was to investigate nanostructured lipid carriers as a strategy to improve the efficacy of PZQ in S. mansoni treatment. We prepared NLC2 and NLC4 by adding seventy percent glycerol monostearate (GMS) as the solid lipid, 30% oleic acid (OA) as the liquid lipid and two surfactant systems containing either soybean phosphatidylcholine/poloxamer (PC/P-407) or phosphatidylcholine/Tween 60 (PC/T60), respectively. The carriers were characterized by nuclear magnetic resonance, differential scanning calorimetry, thermogravimetric analysis and Fourier transform-infrared spectroscopy. The safety profile was evaluated using red cell hemolysis and in vitro cytotoxicity assays. The results showed that the encapsulation of PZQ in NLC2 or NLC4 improved the safety profile of the drug. Treatment efficacy was evaluated on the S. mansoni BH strain. PZQ-NLC2 and PZQ-NLC4 demonstrated an improved efficacy in comparison with free PZQ. The results showed that the intestinal transport of free PZQ and PZQ-NLC2 was similar. However, we observed that the concentration of PZQ absorbed was smaller when PZQ was loaded in NLC4. The difference between the amounts of absorbed PZQ could indicate that the presence of T60 in the nanoparticles (NLC4) increased the rigid lipid matrix, prolonging release of the drug. Both systems showed considerable in vitro activity against S. mansoni, suggesting that these systems may be a promising platform for the administration of PZQ for treating schistosomiasis.en
dc.relation.ispartofJournal of Nanoscience and Nanotechnologypt_BR
dc.publisherAmerican Scientific Publisherspt_BR
dc.identifier.citationJournal Of Nanoscience And Nanotechnology. American Scientific Publishers, v. 15, n. 1, p. 761 - 772, 2015.pt_BR
dc.description.provenanceMade available in DSpace on 2015-06-25T12:53:22Z (GMT). No. of bitstreams: 0 Previous issue date: 2015en
dc.description.provenanceMade available in DSpace on 2015-11-26T15:08:39Z (GMT). No. of bitstreams: 0 Previous issue date: 2015en
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