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Type: Artigo de periódico
Title: Cd144, Cd146 And Vegfr-2 Properly Identify Circulating Endothelial Cell
Author: Flores-Nascimento M.C.
Alessio A.M.
de Andrade Orsi F.L.
Annichino-Bizzacchi J.M.
Abstract: Studies evaluating circulating endothelial cells by flow cytometry are faced by a lack of consensus about the best combination of monoclonal antibodies to be used. The rarity of these cells in peripheral blood, which represent 0.01% of mononuclear cells, drastically increases this challenge. Objective: The aim of this study is to suggest some combinations of markers that would safely and properly identify these cells. Methods: Flow cytometry analysis of circulating endothelial cells was performed applying three different panels composed of different combinations of the CD144, CD146, CD31, CD133, CD45 and anti-Vascular endothelial growth factor receptor-2 antibodies. Results: In spite of the rarity of the events, they were detectable and presented similar inter-person numbers of circulating endothelial cells. Conclusion: The combination of markers successfully identified the circulating endothelial cells in healthy individuals, with the use of three different panels confirming the obtained data as reliable.
Editor: Elsevier Editora Ltda
Rights: aberto
Identifier DOI: 10.1016/j.bjhh.2014.11.014
Date Issue: 2015
Appears in Collections:Unicamp - Artigos e Outros Documentos

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