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|Type:||Artigo de periódico|
|Title:||Cd144, Cd146 And Vegfr-2 Properly Identify Circulating Endothelial Cell|
de Andrade Orsi F.L.
|Abstract:||Studies evaluating circulating endothelial cells by flow cytometry are faced by a lack of consensus about the best combination of monoclonal antibodies to be used. The rarity of these cells in peripheral blood, which represent 0.01% of mononuclear cells, drastically increases this challenge. Objective: The aim of this study is to suggest some combinations of markers that would safely and properly identify these cells. Methods: Flow cytometry analysis of circulating endothelial cells was performed applying three different panels composed of different combinations of the CD144, CD146, CD31, CD133, CD45 and anti-Vascular endothelial growth factor receptor-2 antibodies. Results: In spite of the rarity of the events, they were detectable and presented similar inter-person numbers of circulating endothelial cells. Conclusion: The combination of markers successfully identified the circulating endothelial cells in healthy individuals, with the use of three different panels confirming the obtained data as reliable.|
|Editor:||Elsevier Editora Ltda|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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