Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/85241
Type: Artigo
Title: Metal ions bound to the human milk immunoglobulin A: metalloproteomic approach
Author: Pozzi, Carla Mariane Costa
Braga, Camila Pereira
Vieira, José Cavalcante Souza
Cavecci, Bruna
Queiroz, João Vitor de
Barbosa, Herbert de Souza
Arruda, Marco Aurelio Zezzi
Gozzo, Fabio Cesar
Padilha, Pedro de Magalhães
Abstract: The presence of calcium, iron, and zinc bound to human milk secretory IgA (sIgA) was investigated. The sIgA components were first separated by two-dimensional polyacrylamide gel electrophoresis and then identified by electrospray ionization-tandem mass spectrometry (ESI MS MS). The metal ions were detected by flame atomic absorption spectrometry after acid mineralization of the spots. The results showed eight protein spots corresponding to the IgA heavy chain constant region. Another spot was identified as the transmembrane secretory component. Calcium was bound to both the transmembrane component and the heavy chain constant region, while zinc was bound to the heavy chain constant region and iron was not bound with the identified proteins. The association of a metal ion with a protein is important for a number of reasons, and therefore, the findings of the present study may lead to a better understanding of the mechanisms of action and of additional roles that sIgA and its components play in human milk. © 2014 Elsevier Ltd. All rights reserved.
The presence of calcium, iron, and zinc bound to human milk secretory IgA (sIgA) was investigated. The sIgA components were first separated by two-dimensional polyacrylamide gel electrophoresis and then identified by electrospray ionization-tandem mass sp
Subject: Leite humano
Espectrometria de massa
Metaloproteomica
Imunoglobulina A secretora
Eletroforese
Country: Food chem.
Citation: Food Chemistry. Elsevier Ltd, v. 166, n. , p. 492 - 497, 2015.
Rights: fechado
Fechado
Identifier DOI: 10.1016/j.foodchem.2014.06.040
Address: https://www.sciencedirect.com/science/article/pii/S0308814614009261
Date Issue: 2015
Appears in Collections:IQ - Artigos e Outros Documentos

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