Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/82389
Type: Artigo de periódico
Title: Morphological and growth alterations in Vero cells transformed by cisplatin
Author: Goncalves, EM
Ventura, CA
Yano, T
Macedo, MLR
Genari, SC
Abstract: Cisplatin is an antineoplastic agent used to treat solid tumours, such as ovarian, testicular and bladder tumours. However, studies in vitro and in vivo have shown that cisplatin is mutagenic, genotoxic and tumorigenic in other tissues and organs. In this work, we examined the effect of cisplatin on Vero cells, a fibroblast-like cell line. The morphological characteristics were investigated using phase contrast microscopy, scanning electron microscopy and the actin cytoskeleton was labelled with fluorescein isothiocyanate-phalloidin. Cell proliferation was assessed based on the growth curve. Cultured Vero cells treated with cisplatin showed behavioural and morphological alterations associated with cellular transformation. The transformed cells grew in multilayers and formed cellular aggregates. The proliferation and morphological characteristics of the transformed cells were very different from those of control ones. Since transformed Vero cells showed several characteristics related to neoplastic growth, these cells could be a useful model for studying tumour cells in vitro. (c) 2006 Published by Elsevier Ltd on behalf of International Federation for Cell Biology.
Subject: cellular transformation
cisplatin
in vitro carcinogenesis
Vero cells
Country: Inglaterra
Editor: Academic Press Ltd Elsevier Science Ltd
Rights: fechado
Identifier DOI: 10.1016/j.cellbi.2005.12.007
Date Issue: 2006
Appears in Collections:Unicamp - Artigos e Outros Documentos

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