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|Type:||Artigo de periódico|
|Title:||Modeling of sporicidal effect of hydrogen peroxide in the sterilization of low density polyethylene film inoculated with Bacillus subtilis spores|
|Abstract:||The sporicidal effect of hydrogen peroxide (H(2)O(2)) in the sterilization of low density polyethylene film (LOPE) was evaluated using a Central Composite Design (CCD). The effects of contact time (5-19s), bath temperature (23-70 degrees C) and concentration of H(2)O(2) (0-35%) in an immersion bath were investigated. A 16 cm(2) film surface was evenly inoculated with 100 mu L of the test microorganism Bacillus subtilis var. globigii ATCC9372 spores. The effective H(2)O(2) sporicidal activity was demonstrated at concentrations from 18 to 35% and in a temperature range from 46 to 70 degrees C, resulting in 2-7 decimal reductions of B. subtilis spores. A quadratic mathematical model representative of the action of H(2)O(2) on the B. subtilis spores was developed as a function of concentration, time and temperature. Test specimens sanitized with 28% H(2)O(2) at 60 degrees C for 8 s showed 4 decimal reductions. In the same sterilization procedure, but extending the time to 16 s, this value increased to 7 decimal reductions, demonstrating the efficiency of H(2)O(2) as a function of contact time. The sterilization system tested showed satisfactory performance in the sterilization of LDPE films, being capable of reaching up to 7 decimal reductions of the bacterial spore population. (C) 2011 Elsevier Ltd. All rights reserved.|
Low density polyethylene film
|Editor:||Elsevier Sci Ltd|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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