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Type: Artigo de periódico
Title: Mouse macrophages release a neutrophil chemotactic mediator following stimulation by staphylococcal enterotoxin type A
Author: Desouza, IA
Hyslop, S
Franco-Penteado, CF
Ribeiro-DaSilva, G
Abstract: Objective and Design. To examine the role of macrophages in the neutrophil migration induced by staphylococcal enterotoxin type A (SEA) in mice. Materials and Methods: Peritoneal macrophages were harvested from male Swiss mice pre-treated with thioglycollate. After adhering to plastic tissue culture dishes, the cells were washed and incubated with RPMI or SEA (0.62-2.5 mug/ml) and washed again prior to further incubation with RPMI alone. The medium was then collected, sterilized and assayed for promigratory activity in the mouse peritoneal cavity. Results: Mouse macrophage monolayers stimulated with SEA secreted a thermolabile neutrophil chemotactic component (MNCC-SEA) with a molecular mass > 100 kDa (by ultrafiltration). This release was dose- and lime-dependent and was inhibited by dexamethasone but not by indomethacin or BW755C. Dexamethasone, indomethacin, BWA4C, BW755C, BN52021, cimetidine and SR48968 had no effect on the neutrophil migration induced by MNCC-SEA while capsaicin and SR140333 reduced this phenomenon. Conclusions: Macrophages play a key role in the neutrophil recruitment induced by SEA probably by releasing an MNCC-SEA that presumably induces neutrophil migration via a mechanism mediated by substance P.
Subject: staphylococcal enterotoxin type A
neutrophil migration
neurogenic inflammation
peritoneal cavity
Country: Suíça
Editor: Birkhauser Verlag Ag
Citation: Inflammation Research. Birkhauser Verlag Ag, v. 50, n. 4, n. 206, n. 212, 2001.
Rights: fechado
Identifier DOI: 10.1007/s000110050745
Date Issue: 2001
Appears in Collections:Unicamp - Artigos e Outros Documentos

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