Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/82032
Type: Artigo de periódico
Title: Multiresidue determination of tetracyclines, sulphonamides and chloramphenicol in bovine milk using HPLC-DAD
Author: Mamani, MCV
Reyes, FGR
Rath, S
Abstract: A high-performance liquid chromatographic method for the simultaneous determination of tetracycline, oxytetracycline, chlortetracycline, sulfamethazine, sulfaquinoxaline. sulfamethoxazole and chloramphenicol in milk has been developed. The determination of these antimicrobials was carried out using HPLC-DAD with a C(18) hybrid column and gradient elution with a mobile phase composed of an aqueous phase of 0.075 mol L(-1) sodium acetate, 0.035 mol L(-1) calcium chloride and 0.025 mol L(-1)sodium EDTA, pH 7 and an organic phase of methanol:acetonitrile, 75:25 v/v. Sample preparation involved protein precipitation followed by solid-phase extraction using a polymeric cartridge. The method was validated and applied for the analysis of pasteurised milk samples commercialised in Brazil. The limits of quantitation for all antimicrobials, with the exception of choramphenicol, were below the maximum residue limit, which indicates that the method is appropriate for the determination of these antimicrobials in milk. (C) 2009 Elsevier Ltd. All rights reserved.
Subject: Antimicrobials
Tetracyclines
Sulphonamides
Chloramphenicol
HPLC
Milk
Country: Inglaterra
Editor: Elsevier Sci Ltd
Rights: fechado
Identifier DOI: 10.1016/j.foodchem.2009.04.032
Date Issue: 2009
Appears in Collections:Unicamp - Artigos e Outros Documentos

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