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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleNitrate Reductase- and Nitric Oxide-Dependent Activation of Sinapoylglucose:malate sinapoyltransferase in Leaves of Arabidopsis thalianapt_BR
dc.contributor.authorSantos, PRpt_BR
dc.contributor.authorVitor, SCpt_BR
dc.contributor.authorFrungillo, Lpt_BR
dc.contributor.authorSaviani, EEpt_BR
dc.contributor.authorOliveira, HCpt_BR
dc.contributor.authorSalgado, Ipt_BR
unicamp.authorSantos-Filho, P. R. Vitor, S. C. Frungillo, L. Saviani, E. E. Oliveira, H. C. Salgado, I. State Univ Campinas UNICAMP, Dept Plant Biol, Inst Biol, BR-13083970 Campinas, SP, Brazilpt_BR
dc.subjectArabidopsis thalianapt_BR
dc.subjectNitric oxide (NO)pt_BR
dc.subjectNitrate reductase (NR)pt_BR
dc.subjectSinapic acid esterspt_BR
dc.subjectSinapoylglucose:malate sinapoyltransferase (SMT)pt_BR
dc.subject.wosL-malate Sinapoyltransferasept_BR
dc.subject.wosL Var-sativuspt_BR
dc.subject.wosSerine Carboxypeptidasept_BR
dc.subject.wosPhenylpropanoid Metabolismpt_BR
dc.subject.wosFlavonoid Accumulationpt_BR
dc.subject.wosSecondary Metabolismpt_BR
dc.subject.wosEster Metabolismpt_BR
dc.description.abstractNitrate reductase (NR) activity is necessary for the synthesis of nitric oxide (NO), a key signaling molecule in plants. Here, we investigated the effect of NR deficiency on NO production and phenylpropanoid metabolism of Arabidopsis thaliana leaves. HPLC-mass spectrometry analysis showed that the NR double mutant (nia1 nia2) is deficient in the synthesis of sinapoylmalate (SM), the main phenylpropanoid end-product in wild-type leaves, resulting in accumulation of its precursor sinapoylglucose (SG). While real-time PCR analysis revealed no significant difference at the transcript level, sinapoylglucose:malate sinapoyltransferase (SMT) activity in leaf extracts was reduced in the mutant compared with the wild type. The low levels of SM in nia1 nia2 leaves do not result from the deficient nitrogen incorporation into amino acids, since the recovery of the amino acid content of nia1 nia2 by irrigating the plants with glutamine did not change the metabolic profile of this mutant. In contrast, an increased supply of nitrate stimulated NR activity and NO production, and enhanced SM and decreased SG levels in both genotypes. Nevertheless, sinapic acid esters in nia1 nia2 were not recovered when compared with those detected in the leaves of the wild-type plant. Mutant plants grown in medium supplemented with malate and an NO donor recovered SM to the levels of wild-type leaves. Overall, the results suggest that SMT activity is dependent on the NR-dependent steady-state levels of NO during plant
dc.relation.ispartofPlant And Cell Physiologypt_BR
dc.relation.ispartofabbreviationPlant Cell Physiol.pt_BR
dc.publisherOxford Univ Presspt_BR
dc.identifier.citationPlant And Cell Physiology. Oxford Univ Press, v. 53, n. 9, n. 1607, n. 1616, 2012.pt_BR
dc.sourceWeb of Sciencept_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorship1Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorship1Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorship1Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsordocumentnumberFAPESP [FAPESP]pt
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