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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleNitrite reduction and superoxide-dependent nitric oxide degradation by Arabidopsis mitochondria: Influence of external NAD(P)H dehydrogenases and alternative oxidase in the control of nitric oxide levelspt_BR
dc.contributor.authorWulff, Apt_BR
dc.contributor.authorOliveira, HCpt_BR
dc.contributor.authorSaviani, EEpt_BR
dc.contributor.authorSalgado, Ipt_BR
unicamp.authorWulff, Alfredo Oliveira, Halley Caixeta Saviani, Elzira Elisabeth Salgado, Ione Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Biol Vegetal, BR-13083970 Campinas, SP, Brazilpt_BR
dc.subjectNitric oxidept_BR
dc.subjectNitrite reductionpt_BR
dc.subjectNitric oxide degradationpt_BR
dc.subjectExternal NAD(P)H dehydrogenasespt_BR
dc.subjectAlternative oxidasept_BR
dc.subjectPlant mitochondriapt_BR
dc.subjectArabidopsis thalianapt_BR
dc.subject.wosNitrate Reductasept_BR
dc.subject.wosReversible Inhibitionpt_BR
dc.subject.wosRadical Productionpt_BR
dc.subject.wosRoot Mitochondriapt_BR
dc.description.abstractMitochondria recently have emerged as important sites in controlling NO levels within the cell. In this study, the synthesis of nitric oxide (NO) from nitrite and its degradation by mitochondria isolated from Arabidopsis thaliana were examined. Oxygen and NO concentrations in the reaction medium were measured with specific electrodes. Nitrite inhibited the respiration of isolated A. thaliana mitochondria, in competition with oxygen, an effect that was abolished or potentiated when electron flow occurred via alternative oxidase (AOX) or cytochrome c oxidase (COX), respectively. The production of NO from nitrite was detected electrochemically only under anaerobiosis because of a superoxide-dependent process of NO degradation. Electron leakage from external NAD(P)H dehydrogenases contributed the most to NO degradation as higher rates of Amplex Red-detected H(2)O(2) production and NO consumption were observed in NAD(P)H-energized mitorchondria. Conversely, the NO-insensitive AOX diminished electron leakage from the respiratory chain, allowing the increase of NO half-life without interrupting oxygen consumption. These results show that the accumulation of nitric oxide derived from nitrite reduction and the superoxide-dependent mechanism of NO degradation in isolated A. thaliana mitochondria are influenced by the external NAD(P)H dehydrogenases and AOX, revealing a role for these alternative proteins of the mitochondrial respiratory chain in the control of NO levels in plant cells. (C) 2009 Elsevier Inc. All rights
dc.relation.ispartofNitric Oxide-biology And Chemistrypt_BR
dc.relation.ispartofabbreviationNitric Oxide-Biol. Chem.pt_BR
dc.publisher.citySan Diegopt_BR
dc.publisherAcademic Press Inc Elsevier Sciencept_BR 15pt_BR
dc.identifier.citationNitric Oxide-biology And Chemistry. Academic Press Inc Elsevier Science, v. 21, n. 2, n. 132, n. 139, 2009.pt_BR
dc.sourceWeb of Sciencept_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorship1Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorship1Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsordocumentnumberFAPESP [05/54246-4]pt
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