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Type: Artigo
Title: Nitric oxide modulates eosinophil infiltration in antigen-induced airway inflammation in rats
Author: Ferreira, H.H.A.
Teixeira, C.E.
Bevilacqua, E.
Zanardo, R.C.O.
De Luca, I.M.S.
Gagioti, S.M.
Sannomiya, P.
De Nucci, G.
Antunes, E.
Abstract: The influence of nitric oxide (NO) on eosinophil infiltration into the airways was investigated in rats actively sensitized with ovalbumin. The animals were treated chronically with the NO synthase inhibitor, N-omega-Nitro-L-arginine methyl ester (L-NAME; 75 mu mol rat(-1) day(-1)), for 4 weeks. Bronchoalveolar lavage was performed at 6, 24, 48 and 72 h after intratracheal injection of ovalbumin. Intratracheal challenge of the sensitized rats with ovalbumin caused a significant increase in total leucocyte infiltration in bronchoalveolar lavage fluid both 24 and 48 h post-ovalbumin injection. Neutrophils and eosinophils peaked, respectively, at 24 h (29%) and 48 h (30%) in bronchoalveolar lavage fluid whereas the mononuclear cell did not differ significantly from the counts in non-sensitized rats at any time. At both 6 and 24 h post-ovalbumin injection, the chronic treatment of the animals with L-NAME affected neither the total nor the differential leucocyte content. However, at 48 h post-ovalbumin challenge, the total cell count was reduced by approximately 48% in the L-NAME-treated animals and this was associated with a marked inhibition (81%) of the eosinophil influx. Histological examination of the lungs from these animals (48 h post-ovalbumin challenge) also showed a prominent reduction (69.5%; P < 0.05) of the eosinophil infiltration in the respiratory segments. Our results demonstrate that NO plays a pivotal role in the eosinophil infiltration in airways of actively sensitized rats
Subject: Eosinófilos
Óxido nítrico
Country: Holanda
Editor: Elsevier
Citation: European Journal Of Pharmacology. Elsevier Science Bv, v. 358, n. 3, n. 253, n. 259, 1998.
Rights: fechado
Identifier DOI: 10.1016/S0014-2999(98)00575-5
Date Issue: 1998
Appears in Collections:FCM - Artigos e Outros Documentos

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