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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleOptical tweezers for measuring red blood cell elasticity: application to the study of drug response in sickle cell diseasept_BR
dc.contributor.authorBrandao, MMpt_BR
dc.contributor.authorFontes, Apt_BR
dc.contributor.authorBarjas-Castro, MLpt_BR
dc.contributor.authorBarbosa, LCpt_BR
dc.contributor.authorCosta, FFpt_BR
dc.contributor.authorCesar, CLpt_BR
dc.contributor.authorSaad, STOpt_BR
unicamp.authorState Univ Campinas, UNICAMP, Dept Internal Med, Sao Paulo, Brazil State Univ Campinas, UNICAMP, Haemoctr, Sao Paulo, Brazil State Univ Campinas, UNICAMP, Phys Inst Gleb Wataghin, Sao Paulo, Brazilpt_BR
dc.subjectoptical tweezerspt_BR
dc.subjectsickle cellpt_BR
dc.subjecthydroxyureapt_BR
dc.subjectred blood cell elasticitypt_BR
dc.subjectdeformabilitypt_BR
dc.subject.wosDeformabilitypt_BR
dc.subject.wosErythrocytespt_BR
dc.subject.wosHemoglobinpt_BR
dc.subject.wosHydroxyureapt_BR
dc.description.abstractThe deformability of erythrocytes is a critical determinant of blood flow in microcirculation. By capturing red blood cells (RBC) with optical tweezers and dragging them through a viscous fluid we were able to measure their overall elasticity. We measured, and compared, the RBC deformability of 15 homozygous patients (HbSS) including five patients taking hydroxyurea (HU) for at least 6 months (HbSS/HU), 10 subjects with sickle cell trait (HbAS) and 35 normal controls. Our results showed that the RBC deformability was significantly lower in haemoglobin S (HbS) subjects (HbSS and HbAS), except for HbSS/HU cells, whose deformability was similar to the normal controls. Our data showed that the laser optical tweezers technique is able to detect differences in HbS RBC from subjects taking HU, and to differentiate RBC from normal controls and HbAS, indicating that this is a very sensitive method and can be applied for detection of drug-response in sickle cell disease.pt
dc.relation.ispartofEuropean Journal Of Haematologypt_BR
dc.relation.ispartofabbreviationEur. J. Haematol.pt_BR
dc.publisher.cityCopenhagenpt_BR
dc.publisher.countryDinamarcapt_BR
dc.publisherBlackwell Munksgaardpt_BR
dc.date.issued2003pt_BR
dc.date.monthofcirculationAPRpt_BR
dc.identifier.citationEuropean Journal Of Haematology. Blackwell Munksgaard, v. 70, n. 4, n. 207, n. 211, 2003.pt_BR
dc.language.isoenpt_BR
dc.description.volume70pt_BR
dc.description.issuenumber4pt_BR
dc.description.firstpage207pt_BR
dc.description.lastpage211pt_BR
dc.rightsfechadopt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn0902-4441pt_BR
dc.identifier.wosidWOS:000181831300002pt_BR
dc.identifier.doi10.1034/j.1600-0609.2003.00027.xpt_BR
dc.date.available2014-11-13T17:36:28Z
dc.date.available2015-11-26T16:01:48Z-
dc.date.accessioned2014-11-13T17:36:28Z
dc.date.accessioned2015-11-26T16:01:48Z-
dc.description.provenanceMade available in DSpace on 2014-11-13T17:36:28Z (GMT). No. of bitstreams: 1 WOS000181831300002.pdf: 227725 bytes, checksum: 4aea3a8643dc46cc9f73b63daa13798e (MD5) Previous issue date: 2003en
dc.description.provenanceMade available in DSpace on 2015-11-26T16:01:48Z (GMT). No. of bitstreams: 2 WOS000181831300002.pdf: 227725 bytes, checksum: 4aea3a8643dc46cc9f73b63daa13798e (MD5) WOS000181831300002.pdf.txt: 19685 bytes, checksum: 1a2cbf629e2ceb7f6dffd7fe09ecb8fc (MD5) Previous issue date: 2003en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/81783pt_BR
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/81783
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/81783-
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