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Type: Artigo de periódico
Title: Optimization of Chitosanase Production by Trichoderma koningii sp Under Solid-State Fermentation
Author: da Silva, LCA
Honorato, TL
Franco, TT
Rodrigues, S
Abstract: This study aimed at the optimization of the production of chitosanase in solid culture. Trichoderma koningii sp., an entomopathogenic fungus, was used to produce chitosanase under solid-state fermentation using a mixture of wheat bran and chitosan. The incubation period; addition of moistening water and culture medium composition were optimized. The protocol to extract the enzyme was also optimized. The optimal conditions for chitosanase production by T. koningii were obtained using a mixture of 3.0 g of wheat bran and 1.5 g of chitosan, with the addition of 2.5 mL of moistening water (pH 5.5) and of 2.5 mL of saline solution (pH 5.5) containing NaNO3 (1.0 g/L), (NH4)(2)HPO4 (1.0 g/L), MgSO4.7H(2)O (1.0 g/L), and NaCl (1.0 g/L). Optimal enzyme extraction was carried out adding 20 mL of sodium acetate buffer (200 mM, pH 5.5) at 30 A degrees C under orbital agitation at 150 rpm for 6 min. The optimized production yielded 4.84 IU/gds.
Subject: Solid-state fermentation
Trichoderma koningii
Response surface methodology
Process optimization
Country: EUA
Editor: Springer
Rights: fechado
Identifier DOI: 10.1007/s11947-010-0479-1
Date Issue: 2012
Appears in Collections:Unicamp - Artigos e Outros Documentos

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