Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/81643
Type: Artigo de periódico
Title: Optimising the synthesis of isoamyl butyrate using Rhizopus sp lipase with a central composite rotatable design
Author: Macedo, GA
Pastore, GM
Rodrigues, MI
Abstract: Microbial lipases (E.C. 3.1.1.3) produced by Geotrichum sp. and Rhizopus sp. were prepared and tested as catalysts in the esterification of isoamyl alcohol and butyric acid in a solvent-free system. Response surface methodology (RSM) based on a five-level, two-variable central composite rotatable design (CCRD) was used to optimise this process, with respect to important reaction variables-substrate molar ratio (RM), temperature and lipase concentration (L). The variables RM and L were the most significant in the process. An increase in alcohol concentration from 1:2 to 4:1 caused a decrease in yield to 18.45%, after 48 h of reaction time, and a variation of L from 1 to 10% caused an increase in yield to 32.2%. The optimum conditions found to achieve maximum ester yield (75%) are: RM = 1.5:1; L = 5.5% (w/w), 40 degreesC, after 48 h of reaction time. (C) 2003 Elsevier Ltd. All rights reserved.
Subject: central composite rotatable design (CCRD)
enzymic synthesis
esterification
isoamyl butyrate
Rhizopus sp lipase
Geotrichum sp lipase
response surface methodology (RSM)
Country: Inglaterra
Editor: Elsevier Sci Ltd
Rights: fechado
Identifier DOI: 10.1016/S0032-9592(03)00153-5
Date Issue: 2004
Appears in Collections:Unicamp - Artigos e Outros Documentos

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