Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/81636
Type: Artigo de periódico
Title: Optimisation of germination time and temperature on the concentration of bioactive compounds in Brazilian soybean cultivar BRS 133 using response surface methodology
Author: Paucar-Menacho, LM
Berhow, MA
Mandarino, JMG
de Mejia, EG
Chang, YK
Abstract: The objective was to optimise the effect of germination time and temperature on the concentration of soluble protein. lunasin, 13131, lectin, saponins and isoflavones in soybean seeds from cultivar BRS 133. Isoflavone and saponin concentrations were analysed by HPLC. Lunasin, Bowman-Birk inhibitor and lectin were analysed by ELISA and western blot. The effects of the variations in germination time and temperature on bioactive Compounds were analysed using the response surface methodology (RSM), with a 2(2) central composite rotational design. Germination of soybean for 42 h at 25 degrees C resulted in an increase of 61.7% of lunasin, decrease of 58.7% in lectin and 70.0% in lipoxygenase activity. Optimal increases in the concentrations of isoflavone aglycones were observed in combination of 63 h of germination and 30 degrees C. A significant increase of 32.2% in the concentration of soy saponins was observed in combination of 42 h of germination at 25 degrees C. (C) 2009 Elsevier Ltd. All rights reserved.
Subject: Soybean
Germination
Soy peptides
Lunasin
Bowman-Birk inhibitor
Lectin
Isoflavone
Saponin
Country: Inglaterra
Editor: Elsevier Sci Ltd
Rights: fechado
Identifier DOI: 10.1016/j.foodchem.2009.07.011
Date Issue: 2010
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File Description SizeFormat 
WOS000271971900032.pdf1.03 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.