Please use this identifier to cite or link to this item:
Type: Artigo de periódico
Title: Overexpression of apolipoprotein CIII increases and CETP reverses diet-induced obesity in transgenic mice
Author: Salerno, AG
Silva, TR
Amaral, MEC
Alberici, LC
Bonfleur, ML
Patricio, PR
Francesconi, EPMS
Grassi-Kassisse, DM
Vercesi, AE
Boschero, AC
Oliveira, HCF
Abstract: Objective: We recently described that hypertriglyceridemic apolipoprotein (apo) CIII transgenic mice show increased whole body metabolic rate. In this study, we used these apo CIII-expressing mice, combined or not with the expression of the natural promoter-driven CETP gene, to test the hypothesis that both proteins modulate diet-induced obesity. Measurements and results: Mice expressing apo CIII, CIII/CETP, CETP and nontransgenic (NonTg) mice were maintained on a high-fat diet (14% fat by weight) during 20 weeks after weaning. At the end of this period, all groups exhibited the expected lipemic phenotype. Fasting glucose levels were neither affected by the high-fat diet nor by the distinct genotypes. However, apo CIII mice showed significantly higher glycemia (similar to 35%) and lower insulin levels (similar to 45%) in the fed state, compared with the NonTg mice. The apo CIII mice presented significantly increased body weight, lipid content of the carcass (similar to 25%), visceral adipose tissue mass (about twofold) and adipocyte size (similar to 25%) compared with the CETP and NonTg mice. The CETP expression in the apo CIII background normalized the subcutaneous adipose depot and visceral adipocyte size to the levels of NonTg mice. Plasma leptin levels were lower in CETP groups (25 -50%) and higher in the apo CIII mice. Similar core body temperature in all groups and similar liver mitochondrial resting respiration rates in CIII and NonTg mice indicate no differences in basal energy expenditure rates among these mice fed a high-fat diet. Conclusion: The elevation of plasma apo CIII levels aggravates diet-induced obesity and the expression of physiological levels of circulating CETP reverses this adipogenic effect, indicating a novel role for CETP in modulating adiposity.
Subject: CETP
apolipoprotein CIII
diet-induced obesity
mitochondrial respiration
Country: Inglaterra
Editor: Nature Publishing Group
Citation: International Journal Of Obesity. Nature Publishing Group, v. 31, n. 10, n. 1586, n. 1595, 2007.
Rights: fechado
Identifier DOI: 10.1038/sj.ijo.0803646
Date Issue: 2007
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File Description SizeFormat 
WOS000249737100014.pdf187.96 kBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.