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Type: Artigo de periódico
Title: Localization of dysfunctional tight junctions in Salmonella enterica serovar Typhimurium-infected epithelial layers
Author: Jepson, MA
Schlecht, HB
Collares-Buzato, CB
Abstract: Infection of polarized MDCK epithelial layers by Salmonella enterica serovar Typhimurium is accompanied by increased tight junction permeability and by contraction of perijunctional actinomyosin. We localized dysfunctional tight junctions in serovar Typhimurium-infected MDCK layers by imaging apical-basolateral intramembrane diffusion of fluorescent lipid and found that loss of the apical-basolateral diffusion barrier (tight junction fence function) was most marked in areas of prominent perijunctional contraction. The protein kinase inhibitor staurosporine prevented perijunctional contraction but did not reverse the effects of serovar Typhimurium on tight junction barrier function. Hence, perijunctional contraction is not required for Salmonella-induced tight junction dysfunction and this epithelial response to infection may be multifactorial.
Country: EUA
Editor: Amer Soc Microbiology
Rights: aberto
Identifier DOI: 10.1128/IAI.68.12.7202-7208.2000
Date Issue: 2000
Appears in Collections:Unicamp - Artigos e Outros Documentos

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