Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/81196
Type: Artigo de periódico
Title: Precipitation of clavulanic acid from fermentation broth with potassium 2-ethyl hexanoate salt
Author: Hirata, DB
Oliveira, JHHL
Leao, KV
Rodrigues, MI
Ferreira, AG
Giulietti, M
Barboza, M
Hokka, CO
Abstract: This paper describes a direct precipitation reaction of clavulanic acid (CA) using potassium 2-ethyl hexanoate salt. Clavulanic acid is an important molecule produced by the pharmaceutical industry to overcome problems relating to bacterial resistance to antibiotics. However, precipitation of this organic compound has been little studied and, unlike inorganic compounds, its isolation and precipitation usually involve a complex and meticulous process. The purpose of this work was to improve the purification process and increase the yield of CA from fermented broth by examining the influence of the combined concentrations of clavulanic acid (in organic solvent) and potassium 2-ethyl hexanoate on the potassium clavulanate precipitation reaction. Clavulanic acid was extracted at temperatures below 20 degrees C and preferably close to 15 degrees C in the water-immiscible organic solvent ethyl acetate. The drying step was performed with a suitable desiccant to produce an insoluble salt of potassium clavulanate. The resulting precipitate was crystalline and stable, a finding that was confirmed by an NMR (1)H analysis. (C) 2009 Elsevier B.V. All rights reserved.
Subject: Clavulanic acid
Potassium clavulanate
Precipitation
Potassium 2-ethyl hexanoate
Purification
Country: Holanda
Editor: Elsevier Science Bv
Rights: fechado
Identifier DOI: 10.1016/j.seppur.2009.01.010
Date Issue: 2009
Appears in Collections:Unicamp - Artigos e Outros Documentos

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