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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleLipid chain, dynamics in stratum corneum studied by spin label electron paramagnetic resonancept_BR
dc.contributor.authorAlonso, Apt_BR
dc.contributor.authorMeirelles, NCpt_BR
dc.contributor.authorTabak, Mpt_BR
unicamp.authorUniv Fed Goias, Inst Fis, BR-74001970 Goiania, Go, Brazil Univ Estadual Campinas, Inst Biol, Dept Bioquim, BR-13083970 Campinas, SP, Brazil Univ Sao Paulo, Inst Quim, BR-13560970 Sao Carlos, SP, Brazilpt_BR
dc.subjectstratum corneumpt_BR
dc.subjectmembrane fluiditypt_BR
dc.subjectphase transitionpt_BR
dc.subject.wosEpidermal Permeability Barrierpt_BR
dc.subject.wosIntercellular Membranespt_BR
dc.subject.wosWater Permeabilitypt_BR
dc.description.abstractThe lipid chain motions in stratum corneum (SC) membranes have been studied through electron paramagnetic resonance (EPR) spectroscopy of stearic acid spin-labeled at the 5th, 12th and 16th carbon atom positions of the acyl chain. Lipids have been extracted from SC with a series of chloroform/methanol mixtures, in order to compare the molecular dynamics and the thermotropic behavior in intact Se, lipid-depleted SC (containing covalently bound lipids of the corneocyte envelope) and dispersion of extracted SC lipids. The segmental motion of 5- and 12-doxylstearic acid (5- and 12-DSA) and the rotational correlation time of 16-doxylstearic acid (16-DSA) showed that the envelope lipids are more rigid and the extracted lipids are more fluid than the lipids of the intact SC over the range of temperature measured. The lower fluidity observed for the corneocyte envelope, that may be caused mainly due to lipid-protein interactions, suggests a major contribution of this lipid domain to the barrier function of SC. Changes in the activation energy for reorientational diffusion of the 16-DSA spin label showed apparent phase transitions around 54 degrees C, for the three SC samples. Some lipid reorganization may occur in SC above 54 degrees C, in agreement with results reported from studies with several other techniques. This reorganization is sensitive to the presence of the extractable intercellular lipids, being different in the lipid-depleted sample as compared to native SC and lipid dispersion. The results contribute to the understanding of alkyl chain packing and mobility in the SC membranes, which are involved in the mechanisms that control the permeability of different compounds through skin, suggesting an important involvement of the envelope in the skin barrier. (C) 2000 Elsevier Science Ireland Ltd. All rights
dc.relation.ispartofChemistry And Physics Of Lipidspt_BR
dc.relation.ispartofabbreviationChem. Phys. Lipidspt_BR
dc.publisherElsevier Sci Ireland Ltdpt_BR
dc.identifier.citationChemistry And Physics Of Lipids. Elsevier Sci Ireland Ltd, v. 104, n. 2, n. 101, n. 111, 2000.pt_BR
dc.sourceWeb of Sciencept_BR
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