Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/80951
Type: Artigo de periódico
Title: Pregnancy-induced chromatin remodeling in the breast of postmenopausal women
Author: Russo, J
Santucci-Pereira, J
de Cicco, RL
Sheriff, F
Russo, PA
Peri, S
Slifker, M
Ross, E
Mello, MLS
Vidal, BC
Belitskaya-Levy, I
Arslan, A
Zeleniuch-Jacquotte, A
Bordas, P
Lenner, P
Ahman, J
Afanasyeva, Y
Hallmans, G
Toniolo, P
Russo, IH
Abstract: Early pregnancy and multiparity are known to reduce the risk of women to develop breast cancer at menopause. Based on the knowledge that the differentiation of the breast induced by the hormones of pregnancy plays a major role in this protection, this work was performed with the purpose of identifying what differentiation-associated molecular changes persist in the breast until menopause. Core needle biopsies (CNB) obtained from the breast of 42 nulliparous (NP) and 71 parous (P) postmenopausal women were analyzed in morphology, immunocytochemistry and gene expression. Whereas in the NP breast, nuclei of epithelial cells were large and euchromatic, in the P breast they were small and hyperchromatic, showing strong methylation of histone 3 at lysine 9 and 27. Transcriptomic analysis performed using Affymetrix HG_U133 oligonucleotide arrays revealed that in CNB of the P breast, there were 267 upregulated probesets that comprised genes controlling chromatin organization, transcription regulation, splicing machinery, mRNA processing and noncoding elements including XIST. We concluded that the differentiation process induced by pregnancy is centered in chromatin remodeling and in the mRNA processing reactome, both of which emerge as important regulatory pathways. These are indicative of a safeguard step that maintains the fidelity of the transcription process, becoming the ultimate mechanism mediating the protection of the breast conferred by full-term pregnancy.
Subject: pregnancy
breast differentiation
H3 methylation
noncoding elements
transcription factors
Country: EUA
Editor: Wiley-blackwell
Rights: fechado
Identifier DOI: 10.1002/ijc.27323
Date Issue: 2012
Appears in Collections:Unicamp - Artigos e Outros Documentos

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