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|Title:||Pre-exposure to staphylococcal enterotoxin a exacerbates the pulmonary allergic eosinophil recruitment in rats|
Mello, G.C. de
De Nucci, G.
Souza, I. A. de
|Abstract:||Gram-positive Staphylococcus aureus releases classical enterotoxins which aggravates allergic airway diseases However, little is known about the mechanisms underlying the cell influx exacerbation in asthmatic individuals underexposure to Staphylococcal enterotoxins We therefore aimed to investigate the effects of airways exposure to Staphylococcal enterotoxin A (SEA) to pulmonary leukocyte recruitment in rats sensitized and challenged with ovalbumin (OVA) Rats were exposed to SEA at 4 h prior to OVA challenge or at 4 h post-OVA challenge Bronchoalveolar lavage (BAL) fluid. bone marrow and lung tissue were obtained at 24 11 after OVA challenge Pre-exposure to SEA markedly enhanced the eosinophil counts in both BAL fluid and pulmonary tissue in OVA-challenged rats. whereas neutrophil and mononuclear cell counts remained unchanged In bone marrow, pre-exposure to SEA alone significantly increased the number of eosmophils, and that was further increased in OVA-challenged rats Exposure to SEA post-OVA challenge did not affect the number of eosmophils, neutrophils and mononuclear cells in BAL fluid Pre-exposure to the endotoxin lipopolyssacharide (ITS) in OVA-challenged animals rather enhanced the neutrophil number in BAL fluid In rats pre-exposed to SEA and OVA-challenged, a marked elevation in the levels of TNF-alpha and eotaxin (but not of IL-10) in BAL fluid was observed The eotaxin levels increased by about of 3-fold in alveolar macrophages treated with SEA in vitro In conclusion, airways pre-exposure to SEA causes a selective increase in eosinophil number in BAL fluid and bone marrow of OVA-challenged rats by mechanisms involving enhancement of TNF-alpha and eotaxin synthesis|
|Citation:||International Immunopharmacology. Elsevier Science Bv, v. 10, n. 1, n. 43, n. 49, 2010.|
|Appears in Collections:||FCM - Artigos e Outros Documentos|
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