Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/80930
Type: Artigo de periódico
Title: Pre-exposure to Staphylococcal enterotoxin A exacerbates the pulmonary allergic eosinophil recruitment in rats
Author: Mariano, NS
de Mello, GC
Ferreira, T
Schenka, A
Camargo, EA
de Nucci, G
DeSouza, IA
Antunes, E
Abstract: Gram-positive Staphylococcus aureus releases classical enterotoxins which aggravates allergic airway diseases However, little is known about the mechanisms underlying the cell influx exacerbation in asthmatic individuals underexposure to Staphylococcal enterotoxins We therefore aimed to investigate the effects of airways exposure to Staphylococcal enterotoxin A (SEA) to pulmonary leukocyte recruitment in rats sensitized and challenged with ovalbumin (OVA) Rats were exposed to SEA at 4 h prior to OVA challenge or at 4 h post-OVA challenge Bronchoalveolar lavage (BAL) fluid. bone marrow and lung tissue were obtained at 24 11 after OVA challenge Pre-exposure to SEA markedly enhanced the eosinophil counts in both BAL fluid and pulmonary tissue in OVA-challenged rats. whereas neutrophil and mononuclear cell counts remained unchanged In bone marrow, pre-exposure to SEA alone significantly increased the number of eosmophils, and that was further increased in OVA-challenged rats Exposure to SEA post-OVA challenge did not affect the number of eosmophils, neutrophils and mononuclear cells in BAL fluid Pre-exposure to the endotoxin lipopolyssacharide (ITS) in OVA-challenged animals rather enhanced the neutrophil number in BAL fluid In rats pre-exposed to SEA and OVA-challenged, a marked elevation in the levels of TNF-alpha and eotaxin (but not of IL-10) in BAL fluid was observed The eotaxin levels increased by about of 3-fold in alveolar macrophages treated with SEA in vitro In conclusion, airways pre-exposure to SEA causes a selective increase in eosinophil number in BAL fluid and bone marrow of OVA-challenged rats by mechanisms involving enhancement of TNF-alpha and eotaxin synthesis. (C) 2009 Elsevier B V All rights reserved
Subject: Staphylococcal enterotoxins
Lung inflammation
Eosinophils
Eotaxin
Bone marrow
Country: Holanda
Editor: Elsevier Science Bv
Rights: fechado
Identifier DOI: 10.1016/j.intimp.2009.09.017
Date Issue: 2010
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File Description SizeFormat 
WOS000274361700006.pdf2.48 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.