Please use this identifier to cite or link to this item:
|Type:||Artigo de periódico|
|Title:||LC50 of the peptide produced by the entomopathogenic fungus Nomuraea rileyi (Farlow) Samson active against third instar larvae of Anticarsia gemmatalis (Lep.: Noctuidae)|
de Barros, NM
|Abstract:||The entomopathogenic fungus Nomuraea rileyi (Farlow) Samson produced a peptide active against Anticarsia gemmatalis 3rd instar larvae. To produce this peptide, N. rileyi was cultivated aerobically in Saboraud, maltose, Yeast-extract broth at 26 +/- 1degreesC for 12 days, after which the medium was filtered and separated in a liquid/liquid extractor, concentrated and the peptide purified chromatographically. The crystals obtained were kept refrigerated until needed for LC50 analysis. The LC50 of this peptide against A. gemmatalis 3rd instar larvae was determined in triplicate experiments using solutions containing 1.0, 0.2, 0.1, 0.01, 0.001 and 0.0001 mg/ml of N. rileyi peptide. The results of these experiments were used to calculate a linear equation in which Y = 6,81176 + 1,01382 * LOGx, giving a LC50 value of 0.0163 mg/ml.|
|Editor:||Inst Tecnologia Parana|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.