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Type: Artigo de periódico
Title: LC50 of the peptide produced by the entomopathogenic fungus Nomuraea rileyi (Farlow) Samson active against third instar larvae of Anticarsia gemmatalis (Lep.: Noctuidae)
Author: Onofre, SB
Gonzalez, RR
Messias, CL
Azevedo, JL
de Barros, NM
Abstract: The entomopathogenic fungus Nomuraea rileyi (Farlow) Samson produced a peptide active against Anticarsia gemmatalis 3rd instar larvae. To produce this peptide, N. rileyi was cultivated aerobically in Saboraud, maltose, Yeast-extract broth at 26 +/- 1degreesC for 12 days, after which the medium was filtered and separated in a liquid/liquid extractor, concentrated and the peptide purified chromatographically. The crystals obtained were kept refrigerated until needed for LC50 analysis. The LC50 of this peptide against A. gemmatalis 3rd instar larvae was determined in triplicate experiments using solutions containing 1.0, 0.2, 0.1, 0.01, 0.001 and 0.0001 mg/ml of N. rileyi peptide. The results of these experiments were used to calculate a linear equation in which Y = 6,81176 + 1,01382 * LOGx, giving a LC50 value of 0.0163 mg/ml.
Subject: Anticarsia gemmatalis
Nomuraea rileyi
integrated control
secondary metabolite
Country: Brasil
Editor: Inst Tecnologia Parana
Rights: aberto
Identifier DOI: 10.1590/S1516-89132002000300004
Date Issue: 2002
Appears in Collections:Unicamp - Artigos e Outros Documentos

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