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Type: Artigo de periódico
Title: Inoculum padronization for the production of cutinase by Fusarium oxysporum
Author: Pio, TF
Fraga, LP
Macedo, GA
Abstract: Cutinase is a versatile enzyme showing several interesting properties for application in industrial processes. The widespread use of this enzyme depends on the development of an efficient and low-cost production system. One of the most important steps in a fermentation process is the standardization of the inoculum characteristics. In this study, the production of cutinase by Fusarium oxysporum showed a statistically significant relationship with both the inoculum size and the inoculum PDA pH. The greatest activities were 19.1 U/mL at PDA pH 7.0 and 22.72 U/mL using an aliquot of 12.72 x 10(7) spores/mL. The macroscopic characteristics of the colonies of Fusarium oxysporum changed according to the variation of the medium pH, with the best results recorded in those colonies presenting a cotton white aspect.
Subject: Fusarium oxysporum
macroscopic characteristics
fermentation process
Country: Brasil
Editor: Soc Brasileira Microbiologia
Rights: aberto
Identifier DOI: 10.1590/S1517-83822008000100018
Date Issue: 2008
Appears in Collections:Unicamp - Artigos e Outros Documentos

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