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Type: Artigo de periódico
Title: Inhibition of mitochondrial permeability transition by low pH is associated with less extensive membrane protein thiol oxidation
Author: Teixeira, BMAR
Kowaltowski, AJ
Castilho, RF
Vercesi, AE
Abstract: Ca2+ and inorganic phosphate-induced mitochondrial swelling and membrane protein thiol oxidation, which are associated with mitochondrial permeability transition, are inhibited by progressively decreasing the incubation medium pH between 7.2 and 6.0. Nevertheless, the detection of mitochondrial H2O2 production under these conditions is increased. Permeability transition induced by phenylarsine oxide, which promotes membrane protein thiol cross-linkage in a process independent of Ca2+ or reactive oxygen species, is also strongly inhibited in acidic incubation media. In addition, we observed that the decreased protein thiol reactivity with phenylarsine oxide or phenylarsine oxide-induced swelling at pH 6.0 is reversed by diethyl pyrocarbonate, in a hydroxylamine-sensitive manner. These results provide evidence that the inhibition of mitrochondrial permeability transition observed at lower incubation medium pH is mediated by a decrease in membrane protein thiol reactivity, related to the protonation of protein histidyl residues.
Subject: mitochondrial permeability transition
acid pH
protein sulfhydryl oxidation
reactive oxygen species
Country: EUA
Editor: Kluwer Academic/plenum Publ
Rights: aberto
Identifier DOI: 10.1023/A:1020210827389
Date Issue: 1999
Appears in Collections:Unicamp - Artigos e Outros Documentos

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