Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/80581
Type: Artigo de periódico
Title: Inhibition of human gingival gelatinases (MMP-2 and MMP-9) by metal salts
Author: de Souza, AP
Gerlach, RF
Line, SRP
Abstract: Objectives: The interaction between metal ions and the oral environment is a major subject matter in dental research. Matrix metalloproteinases (MMPs) have been implicated in several pathologic oral processes such as periodontal tissue destruction, root caries, tumour invasion and temporomandibular joint disorders. The aim of this work was to test the effect of Zn, Cu, Sn and Hg ions on the activity of the major gingival gelatinolytic MMPs. Methods: Gingival explants were cultured overnight in DMEM and the activity of secreted enzymes was analyzed by gelatin zymography in buffers containing different metal ion concentrations. The major gelatinolytic proteinases present in the conditioned media were characterized as MMP-2 and MMP-9 by immunoprecipitation with specific antibodies. The electrophoretic bands were scanned and the transmittance values were analyzed with the Sigmagel software (Sigma). Results: ZnSO4 was a strong inhibitor of MMP-2 (I-50 = 15 mu M) and MMP-9 (I-50 = 40 mu M) whereas CuSO4, HgSO4 and SnCl2 showed less efficient inhibition potential. Significance: Our findings show that the activity of oral tissue MMPs may be modulated by metal ions present in the oral environment. Therefore, the accumulation of metals in connective tissue may interfere with the formation and resorption of the extracellular matrix components. (C) 2000 Academy of Dental Materials. Published by Elsevier Science Ltd. All rights reserved.
Subject: gelatinases
MMP-2
MMP-9
metal
zinc
copper
Country: Inglaterra
Editor: Elsevier Sci Ltd
Rights: fechado
Identifier DOI: 10.1016/S0109-5641(99)00084-6
Date Issue: 2000
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File Description SizeFormat 
WOS000085644500005.pdf262.61 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.