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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleREGENERATION OF GARLIC PLANTS (ALLIUM-SATIVUM L, CV CHONAN) VIA CELL-CULTURE IN LIQUID-MEDIUMpt_BR
dc.contributor.authorCID, LPBpt_BR
dc.contributor.authorILLG, RDpt_BR
dc.contributor.authorPIEDRABUENA, AEpt_BR
unicamp.authorUNIV CAMPINAS,IB,DEPT GENET & EVOLUT,CAMPINAS,SP,BRAZILpt_BR
dc.subjectALLIUM SATIVUM Lpt_BR
dc.subjectCELL SUSPENSIONpt_BR
dc.subjectREGENERATIONpt_BR
dc.subjectBULB FORMATIONpt_BR
dc.subject.wosSuspension-culturespt_BR
dc.subject.wosPhotoautotrophic Growthpt_BR
dc.subject.wosCalluspt_BR
dc.subject.wosPhotosynthesispt_BR
dc.description.abstractAiming at the genetic improvement of garlic cultivars, a cell suspension protocol was established which includes the induction of friable callus, establishment of cells in liquid medium, plating, regeneration, and bulb formation. Calluses of various textures from compact to friable and from green to yellowish were obtained by culturing explants excised from inner leaves of garlic bulbs on Marashig-Shoog (MS) medium with 2,4 dichlorophenoxy acetic acid (2,4-D), (1.1 mg/liter [5.0 mu M]), picloram (1.2 mg/liter [5.0 mu M]), and kinetin (2.1 mg/liter [10 mu M]). Friable callus occurred on MS-A contained 2,4-D alone (1.0 mg/liter [4.52 mu M]) and this callus was used to develop cell suspension cultures, which were maintained in liquid MS-B medium with a 2,4-D/benzyl adenine (BA) (0.5 mg/liter [2.25 mu M]: 0.5 mg/liter [2.22 mu M]) ratio. High plating efficiency was obtained on MS-C medium with different naphthalene acetic acid/BA combinations. Regeneration occurred after transfer of the caulogenic mass to MS-C medium containing 10 mg/liter (74.02 mu M) and 20 mg/liter (148.04 mu M) adenine for 60 days, followed by transfer to adenine-free medium. Plantlets transplanted to soil showed normal phenology. Shoots grown on modified MS medium supplemented with indolylbutryic acid (3.0 mg/liter [14.7 mu M]) stimulated bulb formation by 30 days in culture.pt
dc.relation.ispartofIn Vitro Cellular & Developmental Biology-plantpt_BR
dc.relation.ispartofabbreviationIn Vitro Cell. Dev. Biol.-Plantpt_BR
dc.publisher.cityUpper Marlboropt_BR
dc.publisherSoc In Vitro Biologypt_BR
dc.date.issued1994pt_BR
dc.date.monthofcirculationJULpt_BR
dc.identifier.citationIn Vitro Cellular & Developmental Biology-plant. Soc In Vitro Biology, v. 30P, n. 3, n. 150, n. 155, 1994.pt_BR
dc.language.isoenpt_BR
dc.description.volume30Ppt_BR
dc.description.issuenumber3pt_BR
dc.description.firstpage150pt_BR
dc.description.lastpage155pt_BR
dc.rightsfechadopt_BR
dc.sourceWeb of Sciencept_BR
unicamp.cruespUSPpt_BR
dc.identifier.issn1054-5476pt_BR
dc.identifier.wosidWOS:A1994PA62100005pt_BR
dc.date.available2014-12-16T11:32:09Z
dc.date.available2015-11-26T17:29:18Z-
dc.date.accessioned2014-12-16T11:32:09Z
dc.date.accessioned2015-11-26T17:29:18Z-
dc.description.provenanceMade available in DSpace on 2014-12-16T11:32:09Z (GMT). No. of bitstreams: 1 WOSA1994PA62100005.pdf: 1887605 bytes, checksum: 56bbf5284db6e2e984f231da02c9de83 (MD5) Previous issue date: 1994en
dc.description.provenanceMade available in DSpace on 2015-11-26T17:29:18Z (GMT). No. of bitstreams: 2 WOSA1994PA62100005.pdf: 1887605 bytes, checksum: 56bbf5284db6e2e984f231da02c9de83 (MD5) WOSA1994PA62100005.pdf.txt: 21114 bytes, checksum: e024332e494c469ca795db07ee3a3199 (MD5) Previous issue date: 1994en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/80388pt_BR
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/80388
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/80388-
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