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Type: Artigo de periódico
Title: Redox metabolism in Trypanosoma cruzi: Functional characterization of tryparedoxins revisited
Author: Arias, DG
Marquez, VE
Chiribao, ML
Gadelha, FR
Robello, C
Iglesias, AA
Guerrero, SA
Abstract: Tryparedoxins (TXNs) are multipurpose oxidoreductases from trypanosomatids that transfer reducing equivalents from trypanothione to various thiol proteins. In Trypanosoma cruzi, two genes coding for TXN-like proteins have been identified: TXNI, previously characterized as a cytoplasmic protein, and TXNII, a putative tail-anchored membrane protein. In this work, we performed a comparative functional characterization of T. cruzi TXNs. Particularly, we cloned the gene region coding for the soluble version of TXNII for its heterologous expression. The truncated recombinant protein (without its 22 C-terminal transmembrane amino acids) showed TXN activity. It was also able to transfer reducing equivalents from trypanothione, glutathione, or dihydrolipoamide to various acceptors, including methionine sulfoxide reductases and peroxiredoxins. The results support the occurrence and functionality of a second tryparedoxin, which appears as a new component in the redox scenario for T. cruzi. (C) 2013 Elsevier Inc. All rights reserved.
Subject: Trypanosoma
Reactive oxygen species
Free radicals
Country: EUA
Editor: Elsevier Science Inc
Citation: Free Radical Biology And Medicine. Elsevier Science Inc, v. 63, n. 65, n. 77, 2013.
Rights: fechado
Identifier DOI: 10.1016/j.freeradbiomed.2013.04.036
Date Issue: 2013
Appears in Collections:Unicamp - Artigos e Outros Documentos

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