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Type: Artigo de periódico
Title: Influence of environmental conditions on the expression of virulence factors by Listeria monocytogenes and their use in species identification
Author: Lemes-Marques, EG
Yano, T
Abstract: The hemolytic, lecithinase or phosphatidylinositol-specific phospholipase C activities of Listeria monocytogenes can be used to differentiate this pathogenic bacteria from L. innocua, apathogenic, frequently isolated from environmental sources and food. However, the interpretation of these characteristics is problematic because of the variation in the expression of virulence factor, by L. monocytogenes, which can be influenced by environmental conditions. We used a cheap, simple plate assay to monitor this expression in strains obtained from various sources and grown under different culture conditions. The results were increasingly significant and were obtained adding activated charcoal and different salts to the culture media, and in some cases changing the culture temperature, all with a rigorous control on the process of media sterilization. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
Subject: Listeria monocytogenes
activated charcoal
virulence factors
Country: Holanda
Editor: Elsevier Science Bv
Citation: Fems Microbiology Letters. Elsevier Science Bv, v. 239, n. 1, n. 63, n. 70, 2004.
Rights: fechado
Identifier DOI: 10.1016/j.femsle.2004.08.021
Date Issue: 2004
Appears in Collections:Unicamp - Artigos e Outros Documentos

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