Please use this identifier to cite or link to this item:
Type: Artigo de periódico
Title: Regulation of lysine metabolism and endosperm protein synthesis by the opaque-5 and opaque-7 maize mutations
Author: Azevedo, RA
Lea, PJ
Damerval, C
Landry, J
Bellato, CM
Meinhardt, LW
Le Guilloux, M
Delhaye, S
Varisi, VA
Gaziola, SA
Gratao, PL
Toro, AA
Abstract: Two high lysine maize endosperm mutations, opaque-5 (o5) and opaque-7 (o7), were biochemically characterized for endosperm protein synthesis and lysine metabolism in immature seeds. Albumins, globulins, and glutelins, which have a high content of lysine, were shown to be increased in the mutants, whereas zeins, which contain trace concentrations of lysine, were reduced in relation to the wild-type lines B77xB79+ and B37+. These alterations in the storage protein fraction distribution possibly explain the increased concentration of lysine in the two mutants. Using two-dimensional polyacrylamide gel electrophoresis of proteins of mature grains, variable amounts of zein polypeptides were detected and considerable differences were noted between the four lines studied. The analysis of the enzymes involved in lysine metabolism indicated that both mutants have reduced lysine catabolism when compared to their respective wild types, thus allowing more lysine to be available for storage protein synthesis.
Subject: aspartate kinase
lysine 2-oxoglutarate reductase
storage proteins
Country: EUA
Editor: Amer Chemical Soc
Citation: Journal Of Agricultural And Food Chemistry. Amer Chemical Soc, v. 52, n. 15, n. 4865, n. 4871, 2004.
Rights: fechado
Identifier DOI: 10.1021/jf035422h
Date Issue: 2004
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File Description SizeFormat 
WOS000222866000041.pdf96.87 kBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.