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Type: Artigo de periódico
Title: Efficacy of Pentavalent Antimony, Amphotericin B, and Miltefosine in Leishmania amazonensis-Infected Macrophages Under Normoxic and Hypoxic Conditions
Author: Ayres, DC
Pinto, LA
Giorgio, S
Abstract: Recently, our group demonstrated that mouse lesions infected with Leishmania. a amazonensis are hypoxic. Evidence indicates the negative impact of hypoxia on the efficacy of a variety of chemotherapeutic agents against tumors, fungi,. bacteria. and malaria parasites. In the present study, comparison of the effect of antileishmanial drug's oil L. amazonensis-infected macrophages under normoxic and hypoxic Conditions was performed. We compared the effect of 5% oxygen tension with a tension of 21% oxygen on peritoneal murine macrophage cultures infected with the parasite and treated with glucantime, amphotericin B. or miltefosine. Analysis of the infection index (percentage of infected macrophages X number of amastigotes per macrophage, dose-dependent efficacy of drugs, and IC(50) values demonstrated that hypoxia conferred it small, but significant resistance to all 3 antileishmanial drugs. The present finding suggest that in vitro assays under hypoxia should not be neglected ill drug studies.
Country: EUA
Editor: Amer Soc Parasitologists
Citation: Journal Of Parasitology. Amer Soc Parasitologists, v. 94, n. 6, n. 1415, n. 1417, 2008.
Rights: aberto
Identifier DOI: 10.1645/GE-1613.1
Date Issue: 2008
Appears in Collections:Unicamp - Artigos e Outros Documentos

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