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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleRibotyping, Biotyping and Capsular Typing of Haemophilus influenzae Strains Isolated from Patients in Campinas, Southeast Brazilpt_BR
dc.contributor.authorLancellotti, Mpt_BR
dc.contributor.authorde Pace, Fpt_BR
dc.contributor.authorStehling, EGpt_BR
dc.contributor.authorVillares, MCBpt_BR
dc.contributor.authorBrocchi, Mpt_BR
dc.contributor.authorda Silveira, WDpt_BR
unicamp.author.emailwds@unicamp.brpt_BR
unicamp.authorLancellotti, Marcelo de Pace, Fernanda Stehling, Eliana Guedes Brocchi, Marcelo da Silveira, Wanderley Dias Univ Estadual Campinas, Dept Microbiol & Immunol, Inst Biol, Campinas, SP, Brazilpt_BR
unicamp.authorBarisson Villares, Maria Cecilia Univ Estadual Campinas, Dept Clin Pathol, Med Sch Hosp, Campinas, SP, Brazilpt_BR
dc.subjectHaemophilus influenzaept_BR
dc.subjectcharacterizationpt_BR
dc.subjectribotypingpt_BR
dc.subjectserotypept_BR
dc.subjectbiotypept_BR
dc.subject.wosField Gel-electrophoresispt_BR
dc.subject.wosSerotype-b Strainspt_BR
dc.subject.wosHemophilus-influenzaept_BR
dc.subject.wosPcrpt_BR
dc.subject.wosVirulencept_BR
dc.subject.wosChildrenpt_BR
dc.subject.wosDnapt_BR
dc.description.abstractForty-five Haemophilus influenzae strains isolated from patients were characterized based on biochemical characteristics. Their capsular types were determined by polymerase chain reaction (PCR); they were compared, using two molecular methods [ribotyping with a specific DNA probe amplified from the 16S rDNA region from H. influenzae and through restriction fragment length polymorphism (RLFP) of an amplified 16S DNA region]. The strains were better discriminated by the ribotyping technique that used the 16S probe and by the combination of both techniques. Biotypes I and IV were the most common, followed by biotypes VI, VIII and III. Biotypes II and VII were not found. Most of the capsular samples were nontypable (89%), with capsular types a and b found in 2 and 9% of the samples, respectively. We concluded that there is a very close genetic identity among pathogenic and nonpathogenic strains.pt
dc.relation.ispartofBrazilian Journal Of Infectious Diseasespt_BR
dc.relation.ispartofabbreviationBraz. J. Infect. Dis.pt_BR
dc.publisher.citySalvadorpt_BR
dc.publisher.countryBrasilpt_BR
dc.publisherContextopt_BR
dc.date.issued2008pt_BR
dc.date.monthofcirculationOCTpt_BR
dc.identifier.citationBrazilian Journal Of Infectious Diseases. Contexto, v. 12, n. 5, n. 430, n. 437, 2008.pt_BR
dc.language.isoenpt_BR
dc.description.volume12pt_BR
dc.description.issuenumber5pt_BR
dc.description.firstpage430pt_BR
dc.description.lastpage437pt_BR
dc.rightsfechadopt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn1413-8670pt_BR
dc.identifier.wosidWOS:000263374900015pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorship1Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorship1Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsordocumentnumberFAPESP [98/04612-9, 00/01864-9, 00/01882-7]pt
dc.description.sponsordocumentnumberCNPq [300121/90-3]pt
dc.date.available2014-11-17T04:42:46Z
dc.date.available2015-11-26T16:43:25Z-
dc.date.accessioned2014-11-17T04:42:46Z
dc.date.accessioned2015-11-26T16:43:25Z-
dc.description.provenanceMade available in DSpace on 2014-11-17T04:42:46Z (GMT). No. of bitstreams: 1 WOS000263374900015.pdf: 49800 bytes, checksum: 5ba5cfd098d25b2f59b98158366fee8d (MD5) Previous issue date: 2008en
dc.description.provenanceMade available in DSpace on 2015-11-26T16:43:25Z (GMT). No. of bitstreams: 2 WOS000263374900015.pdf: 49800 bytes, checksum: 5ba5cfd098d25b2f59b98158366fee8d (MD5) WOS000263374900015.pdf.txt: 26496 bytes, checksum: b7b54147d8cc6e35faeded4c5c770c94 (MD5) Previous issue date: 2008en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/79848pt_BR
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/79848
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/79848-
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