Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/79848
Type: Artigo de periódico
Title: Ribotyping, Biotyping and Capsular Typing of Haemophilus influenzae Strains Isolated from Patients in Campinas, Southeast Brazil
Author: Lancellotti, M
de Pace, F
Stehling, EG
Villares, MCB
Brocchi, M
da Silveira, WD
Abstract: Forty-five Haemophilus influenzae strains isolated from patients were characterized based on biochemical characteristics. Their capsular types were determined by polymerase chain reaction (PCR); they were compared, using two molecular methods [ribotyping with a specific DNA probe amplified from the 16S rDNA region from H. influenzae and through restriction fragment length polymorphism (RLFP) of an amplified 16S DNA region]. The strains were better discriminated by the ribotyping technique that used the 16S probe and by the combination of both techniques. Biotypes I and IV were the most common, followed by biotypes VI, VIII and III. Biotypes II and VII were not found. Most of the capsular samples were nontypable (89%), with capsular types a and b found in 2 and 9% of the samples, respectively. We concluded that there is a very close genetic identity among pathogenic and nonpathogenic strains.
Subject: Haemophilus influenzae
characterization
ribotyping
serotype
biotype
Country: Brasil
Editor: Contexto
Rights: fechado
Date Issue: 2008
Appears in Collections:Unicamp - Artigos e Outros Documentos

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