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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleEffects of zoledronic acid on odontoblast-like cellspt_BR
dc.contributor.authorBasso, FGpt_BR
dc.contributor.authorTurrioni, APSpt_BR
dc.contributor.authorHebling, Jpt_BR
dc.contributor.authorCosta, CADpt_BR
unicamp.author.emailcasouzac@foar.unesp.brpt_BR
unicamp.authorBasso, F. G. Univ Estadual Campinas, UNICAMP, Piracicaba Sch Dent, BR-13414903 Piracicaba, SP, Brazilpt_BR
unicamp.authorTurrioni, A. P. S. Hebling, J. de Souza Costa, C. A. Univ Estadual Paulista, UNESP, Araraquara Sch Dent, BR-14801903 Araraquara, SP, Brazilpt_BR
dc.subjectBisphosphonatespt_BR
dc.subjectCell culturept_BR
dc.subjectCytotoxicitypt_BR
dc.subject.wosIn-vitropt_BR
dc.subject.wosGene-expressionpt_BR
dc.subject.wosDental-pulppt_BR
dc.subject.wosBisphosphonatespt_BR
dc.subject.wosProliferationpt_BR
dc.subject.wosBiocompatibilitypt_BR
dc.subject.wosOsteonecrosispt_BR
dc.subject.wosFibroblastspt_BR
dc.subject.wosOsteoblastspt_BR
dc.subject.wosInhibitionpt_BR
dc.description.abstractThe aim of the study was to evaluate the effects of a highly potent bisphosphonate, zoledronic acid (ZOL), on cultured odontoblast-like cells MDPC-23. The cells (1.5 x 10(4) cells/cm(2)) were seeded for 48 h in wells of 24-well dished. Then, the plain culture medium (DMEM) was replaced by fresh medium without fetal bovine serum. After 24 h, ZOL (1 or 5 mu M) was added to the medium and maintained in contact with the cells for 24 h. After this period, the succinic dehydrogenase (SDH) enzyme production (cell viability - MTT assay), total protein (TP) production, alkaline phosphatase (ALP) activity, and gene expression (qPCR) of collagen type I (Col-I) and ALP were evaluated. Cell morphology was assessed by SEM. Five mu M ZOL caused a significant decrease in SDH production. Both ZOL concentrations caused a dose-dependent significant decrease in TP production and ALP activity. ZOL also produced discret morphological alterations in the MDPC-23 cells. Regarding gene expression, 1 mu M ZOL caused a significant increase in Col-I expression. Although 5 mu M ZOL did not affect Col-I expression, it caused a significant alteration in ALP expression (ANOVA and Tukey's test, p < 0.05). ZOL presented a dose-dependent cytotoxic effect on the odontoblast-like cells, suggesting that under clinical conditions the release of this drug from dentin could cause damage to the pulpo-dentin complex. (C) 2012 Elsevier Ltd. All rights reserved.pt
dc.relation.ispartofArchives Of Oral Biologypt_BR
dc.relation.ispartofabbreviationArch. Oral Biol.pt_BR
dc.publisher.cityOxfordpt_BR
dc.publisher.countryInglaterrapt_BR
dc.publisherPergamon-elsevier Science Ltdpt_BR
dc.date.issued2013pt_BR
dc.date.monthofcirculationMAYpt_BR
dc.identifier.citationArchives Of Oral Biology. Pergamon-elsevier Science Ltd, v. 58, n. 5, n. 467, n. 473, 2013.pt_BR
dc.language.isoenpt_BR
dc.description.volume58pt_BR
dc.description.issuenumber5pt_BR
dc.description.firstpage467pt_BR
dc.description.lastpage473pt_BR
dc.rightsfechadopt_BR
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policypt_BR
dc.sourceWeb of Sciencept_BR
unicamp.cruespUNESPpt_BR
dc.identifier.issn0003-9969pt_BR
dc.identifier.wosidWOS:000318322000004pt_BR
dc.identifier.doi10.1016/j.archoralbio.2012.09.016pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorship1Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorship1Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsordocumentnumberFAPESP [2009/54722-1, BP DR 2009/52326-1]pt
dc.description.sponsordocumentnumberFAPESP [2009/54722-1, BP.DR: 2009/52326-1]pt
dc.description.sponsordocumentnumberCNPq [301291/2010-1]pt
dc.date.available2014-08-01T18:30:01Z
dc.date.available2015-11-26T18:05:13Z-
dc.date.accessioned2014-08-01T18:30:01Z
dc.date.accessioned2015-11-26T18:05:13Z-
dc.description.provenanceMade available in DSpace on 2014-08-01T18:30:01Z (GMT). No. of bitstreams: 0 Previous issue date: 2013en
dc.description.provenanceMade available in DSpace on 2015-11-26T18:05:13Z (GMT). No. of bitstreams: 0 Previous issue date: 2013en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/79820
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/79820-
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