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Type: Artigo de periódico
Title: Effects of zoledronic acid on odontoblast-like cells
Author: Basso, FG
Turrioni, APS
Hebling, J
Costa, CAD
Abstract: The aim of the study was to evaluate the effects of a highly potent bisphosphonate, zoledronic acid (ZOL), on cultured odontoblast-like cells MDPC-23. The cells (1.5 x 10(4) cells/cm(2)) were seeded for 48 h in wells of 24-well dished. Then, the plain culture medium (DMEM) was replaced by fresh medium without fetal bovine serum. After 24 h, ZOL (1 or 5 mu M) was added to the medium and maintained in contact with the cells for 24 h. After this period, the succinic dehydrogenase (SDH) enzyme production (cell viability - MTT assay), total protein (TP) production, alkaline phosphatase (ALP) activity, and gene expression (qPCR) of collagen type I (Col-I) and ALP were evaluated. Cell morphology was assessed by SEM. Five mu M ZOL caused a significant decrease in SDH production. Both ZOL concentrations caused a dose-dependent significant decrease in TP production and ALP activity. ZOL also produced discret morphological alterations in the MDPC-23 cells. Regarding gene expression, 1 mu M ZOL caused a significant increase in Col-I expression. Although 5 mu M ZOL did not affect Col-I expression, it caused a significant alteration in ALP expression (ANOVA and Tukey's test, p < 0.05). ZOL presented a dose-dependent cytotoxic effect on the odontoblast-like cells, suggesting that under clinical conditions the release of this drug from dentin could cause damage to the pulpo-dentin complex. (C) 2012 Elsevier Ltd. All rights reserved.
Subject: Bisphosphonates
Cell culture
Country: Inglaterra
Editor: Pergamon-elsevier Science Ltd
Citation: Archives Of Oral Biology. Pergamon-elsevier Science Ltd, v. 58, n. 5, n. 467, n. 473, 2013.
Rights: fechado
Identifier DOI: 10.1016/j.archoralbio.2012.09.016
Date Issue: 2013
Appears in Collections:Unicamp - Artigos e Outros Documentos

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