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Type: Artigo de periódico
Title: Effects of veratrine and veratridine on oxygen consumption and electrical membrane potential of isolated rat skeletal muscle and liver mitochondria
Author: Freitas, EMS
Fagian, MM
Hofling, MAD
Abstract: We have previously shown that veratrine, a mixture of alkaloids known as Veratrum alkaloids, produces skeletal muscle toxicity, and there is evidence that veratrine interferes with the energetics of various systems, including cardiomyocytes and synaptosomes. In this work, we explored the effects of veratrine and veratridine, a component of this mixture, in rat skeletal muscle mitochondria and compared the results with those seen in liver mitochondria. Veratrine and veratridine alkaloids caused a significant concentration-dependent decrease in the rate of state 3 respiration, respiratory control (RCR) and ADP/O ratios in isolated rat skeletal muscle mitochondria (RMM), but not in rat liver mitochondria (RLM) supported by either NADH-linked substrates or succinate. The oxygen consumption experiments showed that RMM were more susceptible to the toxic action of Veratrum alkaloids than RLM. The addition of veratrine (250 mu g/ml) to RMM caused dissipation of the mitochondrial electrical membrane potential during succinate oxidation, but this effect was totally reversed by adding ATP. These results indicate that there are chemical- and tissue-specific toxic effects of veratrine and veratridine on mitochondrial respiratory chain complexes. Identification of the specific respiratory chain targets involved should provide a better understanding of the molecular mechanisms of the toxicity of these agents. (c) 2006 Elsevier Ltd. All rights reserved.
Subject: veratrine
isolated mitochondria
oxygen consumption
mitochondrial electrical membrane potential
respiratory chain complex
Country: Inglaterra
Editor: Pergamon-elsevier Science Ltd
Citation: Toxicon. Pergamon-elsevier Science Ltd, v. 47, n. 7, n. 780, n. 787, 2006.
Rights: fechado
Identifier DOI: 10.1016/j.toxicon.2006.02.009
Date Issue: 2006
Appears in Collections:Unicamp - Artigos e Outros Documentos

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