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Type: Artigo de periódico
Title: Effects of ulipristal acetate on sperm DNA fragmentation during in vitro incubation
Author: Munuce, MJ
Cicare, J
Zumoffen, C
Caille, A
Ghersevich, S
Bahamondes, L
Abstract: Objective Ulipristal acetate (UPA) acts as an emergency contraceptive by inhibiting ovulation. This study explores possible additional effects on the fragmentation of sperm DNA during in vitro incubation. Methods Motile spermatozoa from healthy donors were selected by swim-up and incubated under capacitating conditions in control medium or with UPA (1, 10, 100, 1,000 or 10,000 ng/ml). In some experiments, 200 mu M of H2O2 were added to induce oxidative stress. The sperm chromatin dispersion test was performed to analyse DNA integrity (400 cells; 1000X). Lipid peroxidation (thiobarbituric acid assay), induced-acrosome reaction (AR) and sperm vitality (Eosin Y) were also evaluated in spermatozoa exposed to UPA and/or H2O2. Results During sperm incubation, the percentage of fragmented DNA increased significantly, from 15.0 +/- 1.3 to 41.0 +/- 4.5% (p < 0.001). In the presence of UPA, DNA fragmentation decreased significantly (p < 0.05), in a dose-dependent manner. At 100 and 1000 ng/ml, UPA also counteracted the effect of H2O2 and prevented DNA fragmentation. No effect on sperm vitality, lipid peroxidation or induced-AR was found with any treatment. Conclusions During in vitro sperm capacitation DNA fragmentation increased but the latter was counteracted in the presence of UPA, which possibly acted as a scavenger of reactive oxygen species produced by spermatozoa.
Subject: Spermatozoa
Ulipristal acetate
Sperm chromatin dispersion test
DNA fragmentation
Country: Inglaterra
Editor: Informa Healthcare
Citation: European Journal Of Contraception And Reproductive Health Care. Informa Healthcare, v. 18, n. 5, n. 355, n. 363, 2013.
Rights: fechado
Identifier DOI: 10.3109/13625187.2013.813930
Date Issue: 2013
Appears in Collections:Unicamp - Artigos e Outros Documentos

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