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Type: Artigo de periódico
Title: eae-negative attaching and effacing Escherichia coli from piglets with diarrhea
Author: Penteado, AS
Aidar, L
de Castro, AFP
Yamada, A
Andrade, JRC
Blanco, J
Blanco, M
Blanco, JE
Abstract: One hundred and ninety strains of Escherichia toll that were isolated from pigs with diarrhea in the state of Sao Paulo, Brazil, and that were negative for enterotoxins and cytotoxins were investigated. Strains which adhered to HeLa cells were examined for fluorescence actin staining (FAS), the ability to induce attaching and effacing (A/E) lesions on HEp-2 cells detectable by transmission electron microscopy and the presence of eae gene sequences detected by PCR. Intimin production was detected by western blot and serogrouping was performed. Forty-seven isolates adhered to HeLa cells in several patterns, but none adhered in a localized adherence pattern. However, seven of the 47 adherent strains were positive for the FAS reaction, although the reactions were usually weak or atypical. One FAS-negative and three FAS-positive strains, which were examined for their ability to induce A/E lesions, were all positive. Subsequently, testing of these strains for the ene gene showed that they all lacked this gene. These findings, along with earlier reports of eae-negative A/E E. coli, suggest that higher quantities of E. coli in this category might be detected if more reliance were placed on phenotypic tests rather than on gene detection tests alone. (C) 2001 Editions scientifiques et medicales Elsevier SAS.
Subject: Escherichia coli, porcine
phenotypic and genotypic properties
eae gene
Country: França
Editor: Editions Scientifiques Medicales Elsevier
Citation: Research In Microbiology. Editions Scientifiques Medicales Elsevier, v. 152, n. 1, n. 75, n. 81, 2001.
Rights: fechado
Date Issue: 2001
Appears in Collections:Unicamp - Artigos e Outros Documentos

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