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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleStructural and kinetic characterization of a maize aldose reductasept_BR
dc.contributor.authorde Sousa, SMpt_BR
dc.contributor.authorRosselli, LKpt_BR
dc.contributor.authorKiyota, Ept_BR
dc.contributor.authorda Silva, JCpt_BR
dc.contributor.authorSouza, GHMFpt_BR
dc.contributor.authorPeroni, LApt_BR
dc.contributor.authorStach-Machado, DRpt_BR
dc.contributor.authorEberlin, MNpt_BR
dc.contributor.authorSouza, APpt_BR
dc.contributor.authorKoch, KEpt_BR
dc.contributor.authorArruda, Ppt_BR
dc.contributor.authorTorriani, ILpt_BR
dc.contributor.authorYunes, JApt_BR
unicamp.authorYunes, Jose Andres Ctr Infantil Boldrini, Lab Biol Mol, BR-13083210 Campinas, SP, Brazilpt_BR
unicamp.authorde Sousa, Sylvia Morais Rosselli, Luciana K. Kiyota, Eduardo Souza, Anete P. Arruda, Paulo Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, Dept Genet & Evolucao, Inst Biol, Campinas, SP, Brazilpt_BR
unicamp.authorda Silva, Julio Cesar Torriani, Iris L. Lab Nacl Luz Sincrotron, Campinas, SP, Brazilpt_BR
unicamp.authorda Silva, Julio Cesar Torriani, Iris L. Univ Estadual Campinas, Inst Fis Gleb Wataghin, Campinas, SP, Brazilpt_BR
unicamp.authorSouza, Gustavo H. M. F. Eberlin, Marcos N. Univ Estadual Campinas, Inst Quim, Lab ThoMSon Espectrometria Massas, Campinas, SP, Brazilpt_BR
unicamp.authorPeroni, Luis Antonio Stach-Machado, Dagmar R. Univ Estadual Campinas, Lab Imunol Aplicada, Dept Microbiol & Imunol, Campinas, SP, Brazilpt_BR
unicamp.authorKoch, Karen E. Univ Florida, Dept Horticultural Sci, Gainesville, FL USApt_BR
dc.subjectZea mayspt_BR
dc.subjectAldose reductasept_BR
dc.subjectSmall angle X-ray scatteringpt_BR
dc.subjectCircular dichroism spectroscopypt_BR
dc.subjectMass spectrometrypt_BR
dc.subject.wosSorbitol-dehydrogenase Expressionpt_BR
dc.subject.wosSite-directed Mutagenesispt_BR
dc.description.abstractThe aldo-keto reductases (AKRs) are classified as oxidoreductases and are found in organisms from prokaryotes to eukaryotes. The AKR superfamily consists of more than 120 proteins that are distributed throughout 14 families. Very few plant AKRs have been characterized and their biological functions remain largely unknown. Previous work suggests that AKRs; may participate in stress tolerance by detoxifying reactive aldehyde species. In maize endosperm, the presence of an aldose reductase (AR; EC enzyme has also been hypothesized based on the extensive metabolism of sorbitol. This manuscript identifies and characterizes an AKR from maize (Zea mays L.) with features of an AR. The cDNA clone, classified as AKR4C7, was expressed as a recombinant His-tag fusion protein in Escherichia coli. The product was purified by immobilized metal affinity chromatography followed by anion exchange chromatography. Circular dichroism spectrometry and SAXS analysis indicated that the AKR4C7 protein was stable, remained folded throughout the purification process, and formed monomers of a globular shape, with a molecular envelope similar to human AR. Maize AKR4C7 could utilize DL-glyceraldehyde and some pentoses as substrates. Although the maize AKR4C7 was able to convert sorbitol to glucose, the low affinity for this substrate indicated that AKR4C7 was probably a minimal contributor to sorbitol metabolism in maize seeds. Polyclonal antisera raised against AKR4C7 recognized at least three AR-like polypeptides in maize kernels, consistent with the presence of a small gene family. Diverse functions may have evolved for maize AKRs in association with specific physiological requirements of kernel development. (C) 2008 Elsevier Masson SAS. All rights
dc.relation.ispartofPlant Physiology And Biochemistrypt_BR
dc.relation.ispartofabbreviationPlant Physiol. Biochem.pt_BR
dc.publisherElsevier France-editions Scientifiques Medicales Elsevierpt_BR
dc.identifier.citationPlant Physiology And Biochemistry. Elsevier France-editions Scientifiques Medicales Elsevier, v. 47, n. 2, n. 98, n. 104, 2009.pt_BR
dc.sourceWeb of Sciencept_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorship1Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorship1Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
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