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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleStroma-mediated granulocyte-macrophage colony-stimulating factor (GM-CSF) control of myelopoiesis: spatial organisation of intercellular interactionspt_BR
dc.contributor.authorBorojevic, Rpt_BR
dc.contributor.authorCarvalho, MApt_BR
dc.contributor.authorCorrea-Junior, JDpt_BR
dc.contributor.authorArcanjo, Kpt_BR
dc.contributor.authorGomes, Lpt_BR
dc.contributor.authorJoazeiro, PPpt_BR
dc.contributor.authorBalduino, Apt_BR
dc.contributor.authorWettreich, Apt_BR
dc.contributor.authorCoelho-Sampaio, Tpt_BR
unicamp.authorUniv Fed Rio de Janeiro, Dept Histol Embriol, BR-21941970 Rio De Janeiro, Brazil Univ Fed Rio de Janeiro, Inst Ciencias Biomed, Dept Bioquim, BR-21941970 Rio De Janeiro, Brazil Univ Fed Rio de Janeiro, Hosp Univ Clementino Fraga Filho, Programa Avancado Biol Celular Aplicada & Med, BR-21941970 Rio De Janeiro, Brazil Univ Estadual Campinas, Dept Biol Celular, BR-13083970 Campinas, SP, Brazil Univ Estadual Campinas, Dept Histol, BR-13083970 Campinas, SP, Brazilpt_BR
dc.subjecthaemopoiesispt_BR
dc.subjectgranulocyte-macrophage colony-stimulating factorpt_BR
dc.subjectmyelopoietic stromapt_BR
dc.subjectcationic ferritinpt_BR
dc.subjectproteoglycanspt_BR
dc.subjectcell culture (murine)pt_BR
dc.subject.wosHeparan-sulfate Proteoglycanspt_BR
dc.subject.wosCell-linept_BR
dc.subject.wosInflammatory Reactionspt_BR
dc.subject.wosGrowth-factorspt_BR
dc.subject.wosGlycosaminoglycanspt_BR
dc.subject.wosProliferationpt_BR
dc.subject.wosExpressionpt_BR
dc.subject.wosCapacitypt_BR
dc.subject.wosReceptorpt_BR
dc.subject.wosMembranept_BR
dc.description.abstractGranulocyte-macrophage colony-stimulating factor (GM-CSF) is one of the major cytokines involved in control of haemopoiesis both in bone marrow and in extramedullar sites. Its biological activity depends upon the composition and physicochemical properties of the microenvironment provided by the supporting stroma. GM-CSF activity is modulated and controlled by the stromal heparan-sulphate proteoglycans, but their optimal interaction occurs only at low pH. We questioned whether the microenvironment organisation of the interface between stroma and haemopoietic cells provides such conditions. We studied myeloid progenitor proliferation in contact with bone marrow-derived and extramedullar stromas using electron microscopy and selective labelling of pericellular components. We present evidence that, upon interaction, the two cell types reorganise their interface both in shape and molecular composition. Haemopoietic cells extend projections that considerably increase the area of intercellular contact, and stromal cells form lamellipodia and carry out a redistribution of membrane-associated sialylated glycoconjugates and proteoglycans. Such rearrangements lead to extensive capping of negatively charged molecules at the interface between the supporting stroma and the haemopoietic cells, leading potentially to a local decrease in pH. Our results indicate that the distribution of negative charges at the cellular interface may be responsible for the selectivity of cell response to GM-CSF.pt
dc.relation.ispartofCell And Tissue Researchpt_BR
dc.relation.ispartofabbreviationCell Tissue Res.pt_BR
dc.publisher.cityNew Yorkpt_BR
dc.publisher.countryEUApt_BR
dc.publisherSpringer-verlagpt_BR
dc.date.issued2003pt_BR
dc.date.monthofcirculationJULpt_BR
dc.identifier.citationCell And Tissue Research. Springer-verlag, v. 313, n. 1, n. 55, n. 62, 2003.pt_BR
dc.language.isoenpt_BR
dc.description.volume313pt_BR
dc.description.issuenumber1pt_BR
dc.description.firstpage55pt_BR
dc.description.lastpage62pt_BR
dc.rightsfechadopt_BR
dc.rights.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0pt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn0302-766Xpt_BR
dc.identifier.wosidWOS:000184839000006pt_BR
dc.identifier.doi10.1007/s00441-003-0726-0pt_BR
dc.date.available2014-11-17T11:04:15Z
dc.date.available2015-11-26T17:29:43Z-
dc.date.accessioned2014-11-17T11:04:15Z
dc.date.accessioned2015-11-26T17:29:43Z-
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dc.description.provenanceMade available in DSpace on 2015-11-26T17:29:43Z (GMT). No. of bitstreams: 2 WOS000184839000006.pdf: 572810 bytes, checksum: 567af7b2c54da4fbcf1d2a7b2619afcf (MD5) WOS000184839000006.pdf.txt: 34216 bytes, checksum: a944277e6632e19f43cdfa629e177395 (MD5) Previous issue date: 2003en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/79592pt_BR
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/79592
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/79592-
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