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Type: Artigo de periódico
Title: Determination of phenolic acids in coffee by micellar electrokinetic chromatography
Author: Risso, EM
Peres, RG
Amaya-Farfan, J
Abstract: A seven-minute micellar electrokinetic chromatography analytical procedure capable of resolving the five principal phenolic acids in coffee, including the isomers of 5-caffcoylquinic acid (5-CQA), 4-CQA and 3-CQA, caffeic acid and ferulic acid is reported. The electrophoretic conditions consisted of an SDS (70 mM)-phosphate (17.6 mM)-methanol (5% v/v) buffer system, pH 2.5, 22.1 degrees C, -17 kV and detection at 325 nm. The Joule effect and the possible interactions between the buffer components and temperature with respect to peak quality, resolution and selectivity were assessed in the concentration range of 25-900 mu g/mL. Performance evaluation of the system used a 3(3-7) factorial design at the 95% confidence level. The lowest correlation coefficient for linearity was 0.99888 for the 4-CQA. Limits of detection and quantification were 0.98 and 4 mu g/mL. The method was tested with both green and roasted coffee beans using four systems of extraction. (c) 2007 Elsevier Ltd. All rights reserved.
Subject: HPCE
capillary electrophoresis
phenolic acids
chlorogenic acid
ferulic acid
caffeic acid
Country: Inglaterra
Editor: Elsevier Sci Ltd
Citation: Food Chemistry. Elsevier Sci Ltd, v. 105, n. 4, n. 1578, n. 1582, 2007.
Rights: fechado
Identifier DOI: 10.1016/j.foodchem.2007.03.028
Date Issue: 2007
Appears in Collections:Unicamp - Artigos e Outros Documentos

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