Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/79578
Type: Artigo de periódico
Title: DETERMINATION OF PHENOBARBITAL IN HUMAN PLASMA BY A SPECIFIC LIQUID CHROMATOGRAPHY METHOD: APPLICATION TO A BIOEQUIVALENCE STUDY
Author: Dalmora, SL
Sangoi, MD
Nogueira, DR
D'Avila, FB
Moreno, RA
Sverdloff, CE
de Oliveira, RA
Borges, NC
Abstract: A liquid chromatography method was developed and validated for the determination of phenobarbital in human plasma using phenytoin as internal standard. The drugs were extracted from plasma by liquid-liquid extraction and separated isocratically on a C(12) analytical column, maintained at 35 degrees C, with water: acetonitrile: methanol (58.8:15.2:26, v/v/v) as mobile phase, run at a flow rate of 1.2 mL/min with detection at 205 nm. The method was linear in the range of 0.1-4 mu g/mL (r(2)=0.9999) and demonstrated acceptable results for the precision, accuracy and stability studies. The method was successfully applied for the bioequivalence study of two tablet formulations (test and reference) of phenobarbital 100 mg after single oral dose administration to healthy human volunteers.
Subject: human plasma
liquid chromatography
phenobarbital
Country: Brasil
Editor: Soc Brasileira Quimica
Rights: aberto
Identifier DOI: 10.1590/S0100-40422010000100023
Date Issue: 2010
Appears in Collections:Unicamp - Artigos e Outros Documentos

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