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Type: Artigo de periódico
Title: Determination of memantine in human plasma by liquid chromatography-electrospray tandem mass spectrometry: Application to a bioequivalence study
Author: Almeida, AA
Campos, DR
Bernasconi, G
Calafatti, S
Barros, FAP
Eberlin, MN
Meurer, EC
Paris, EG
Pedrazzoli, J
Abstract: A simple, sensitive and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of memantine (1) in human plasma is presented. Sample preparation consisted of the addition of amantadine (11) as internal standard (IS), liquid-liquid extraction in basic conditions using a mixture of diethyl ether-chloroform (7:3, v/v) as extracting solvent, followed by centrifugation, solvent evaporation and sample reconstitution in methanol. Both I and 11 (internal standard) were analyzed using a C18 column and a mobile phase composed of methanol-water-formic acid (80:20:0.1, v/v/v). Eluted compounds were monitored using positive mode electrospray (ES) tandem mass spectrometry. The analyses were carried out by selected reaction monitoring (SRM) using the parent to daughter combinations of m/z 180 > 163 (memantine) and m/z 152 > 135 (amantadine). The peak areas from the analyte and IS were used for quantification of I. The achieved limit of quantification (LOQ) was 0.1 ng/mL; the assay exhibited a linear dynamic range of 0.1-50.0 ng/mL with a determination coefficient (r(2)) of at least 0.98. Validation results on linearity, specificity, accuracy, precision and stability, as well as on application to the analysis of samples taken up to 320 h after oral administration of 20 mg (two 10 mg capsules) of I in healthy volunteers demonstrated the applicability to bioequivalence studies. (c) 2006 Elsevier B.V. All rights reserved.
Subject: mass spectrometry
Country: Holanda
Editor: Elsevier Science Bv
Citation: Journal Of Chromatography B-analytical Technologies In The Biomedical And Life Sciences. Elsevier Science Bv, v. 848, n. 2, n. 311, n. 316, 2007.
Rights: fechado
Identifier DOI: 10.1016/j.jchromb.2006.10.045
Date Issue: 2007
Appears in Collections:Unicamp - Artigos e Outros Documentos

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