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Type: Artigo de periódico
Title: Stress-induced alteration in the lipolytic response to beta-adrenoceptor agonists in rat white adipocytes
Author: Farias-Silva, E
Grassi-Kassisse, DM
Wolf-Nunes, V
Spadari-Bratfisch, RC
Abstract: We analysed the sensitivity to beta-adrenoceptor agonists in epididymal adipose cells from rats submitted to a stress protocol previously reported to induce alterations in sensitivity to catecholamines in cardiac tissue from rats. Food intake and body weight were lower, whereas adipocytes basal Lipolysis was higher (control: 0.59 +/- 0.04; stress: 1.00 +/- 0.11, mu mol glycerol/100 mg total lipids/100 min) in stressed compared to control rats. The responses to isoprenaline (pD(2) control: 7.46 +/- 0.11; stress: 8.11 +/- 0.17), adrenaline (pD(2) control: 5.78 +/- 0.20; stress: 6.13 +/- 0.18), and salbutamol (pB(2) control: 5.64 +/- 0.28; stress: 5.92 +/- 0.34) were sensitized, and the lipolytic responses to norepinephrine (pD(2) control: 6.98 +/- 0.13; stress: 6.41 +/- 0.12) and to BRL37344 (pD(2) control: 8.43 +/- 0.19; stress: 7.54 +/- 0.21) were desensitized. Responses to the higher concentration (100 mu M) of isoprenaline (control: 1.80 +/- 0.18; stress: 2.24 +/- 0.10 mu mol glycerol/100 mg total lipids/100 min), epinephrine (control: 1.64 +/- 0.17; stress: 2.24 +/- 0.14 mu mol glycerol/100 mg total Lipids/100 min), salbutamol (control: 0.65 +/- 0.11; stress: 1.21 +/- 0.41 mu mol glycerol/100 mg total lipids/100 min), and d-butyryl-cAMP (control: 1.59 +/- 0.17; stress: 2.72 +/- 0.25) were significantly enhanced in adipocytes from stressed rats, pD(2) or maximum response to CGP12177 were not altered. Supersensitivity to isoprenaline was abolished by 50 nM ICI118,551 but was not modified by 100 nM metoprolol. However, subsensitivity to norepinephrine and to BRL37344 was abolished by 100 nM metoprolol.[il Our results suggest that in epididymal adipocytes from stressed rats there is a desensitization of the response to adrenoceptor agonists mediated by beta(2)-adrenoceptors together with a sensitization of the response mediated by Pn-adrenoceptors. PB-adrenoceptors seem to be resistant to the stress effect.
Subject: adrenergic receptors
footshock stress
epididymal adipocytes
Country: EUA
Editor: Lipid Research Inc
Citation: Journal Of Lipid Research. Lipid Research Inc, v. 40, n. 9, n. 1719, n. 1727, 1999.
Rights: fechado
Date Issue: 1999
Appears in Collections:Unicamp - Artigos e Outros Documentos

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