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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleDepyrogenation of snake antivenom serum solutions by hollow fiber-based pseudobioaffinity filtrationpt_BR
dc.contributor.authorAcconci, Cpt_BR
dc.contributor.authorLegallais, Cpt_BR
dc.contributor.authorVijayalakshmi, Mpt_BR
dc.contributor.authorBueno, SMApt_BR
unicamp.authorUniv Estadual Campinas, Fac Engn Quim, Dept Proc Biotecnol, BR-13083970 Campinas, SP, Brazil Univ Technol Compiegne, CNRS, UMR 6600, F-60205 Compiegne, France Univ Technol Compiegne, Lab Interact Mol & Technol Separat, F-60205 Compiegne, Francept_BR
dc.subjectdepyrogenationpt_BR
dc.subjectendotoxinspt_BR
dc.subjectsnake antivenom horse serumpt_BR
dc.subjecthistidinept_BR
dc.subjectaffinity membranespt_BR
dc.subject.wosImmunoglobulin-gpt_BR
dc.subject.wosMonoclonal-antibodiespt_BR
dc.subject.wosL-histidinept_BR
dc.subject.wosRemovalpt_BR
dc.subject.wosMembranespt_BR
dc.subject.wosEndotoxinpt_BR
dc.subject.wosSeparationpt_BR
dc.subject.wosHistaminept_BR
dc.subject.wosAdsorberspt_BR
dc.description.abstractThe affinity filtration technique using histidine as the pseudobiospecific ligand immobilized on poly(ethylene vinyl alcohol) hollow fiber membranes (Histidine-PEVA) was used to remove endotoxin (ET) from snake antivenom serum solutions. Immobilized histidine bound to horse antibodies (F(ab')(2) fragments) and ET. The effects of solution conditions on the efficiency of ET removal and protein recovery were studied. The lowest antibody adsorption capacity was obtained with acetate pH 6.0 and phosphate pH 6.5 buffers. Under both conditions, ET was removed but the highest efficiency was found for the acetate buffer. This buffer was used to determine the optimal combination of ET concentration and Q(F)/Q(i) (filtrate flow rate/inlet flow rate) ratio for achieving a high rate of ET removal without a significant loss of antibodies. Removal of 93% of the ET and recuperation of 95% of the antibodies were obtained at a Q(F)/Q(i) ratio equal to 0.68 and an initial ET concentration of 675 EU/ml, A high clearance rate of 99% was obtained with initial concentrations of 65 and 351 EU/ml for lachesis-bothrops and bothrops antivenom sera, respectively, and an almost complete depyrogenation of bothrops antivenom serum was achieved, since the final ET content in solution was 0.8 EU/mL. (C) 2000 Elsevier Science B.V. All rights reserved.pt
dc.relation.ispartofJournal Of Membrane Sciencept_BR
dc.relation.ispartofabbreviationJ. Membr. Sci.pt_BR
dc.publisher.cityAmsterdampt_BR
dc.publisher.countryHolandapt_BR
dc.publisherElsevier Science Bvpt_BR
dc.date.issued2000pt_BR
dc.date.monthofcirculation42186pt_BR
dc.identifier.citationJournal Of Membrane Science. Elsevier Science Bv, v. 173, n. 2, n. 235, n. 245, 2000.pt_BR
dc.language.isoenpt_BR
dc.description.volume173pt_BR
dc.description.issuenumber2pt_BR
dc.description.initialpage235pt_BR
dc.description.lastpage245pt_BR
dc.rightsfechadopt_BR
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policypt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn0376-7388pt_BR
dc.identifier.wosidWOS:000087541700009pt_BR
dc.identifier.doi10.1016/S0376-7388(00)00377-Xpt_BR
dc.date.available2014-12-02T16:28:15Z
dc.date.available2015-11-26T16:27:55Z-
dc.date.accessioned2014-12-02T16:28:15Z
dc.date.accessioned2015-11-26T16:27:55Z-
dc.description.provenanceMade available in DSpace on 2014-12-02T16:28:15Z (GMT). No. of bitstreams: 1 WOS000087541700009.pdf: 217529 bytes, checksum: 8ac85746569fad0564ffa9bf059a2e07 (MD5) Previous issue date: 2000en
dc.description.provenanceMade available in DSpace on 2015-11-26T16:27:55Z (GMT). No. of bitstreams: 2 WOS000087541700009.pdf: 217529 bytes, checksum: 8ac85746569fad0564ffa9bf059a2e07 (MD5) WOS000087541700009.pdf.txt: 38808 bytes, checksum: 09ec24d99273225e47bf6d7fbbb3ef04 (MD5) Previous issue date: 2000en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/79515pt_BR
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/79515
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/79515-
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