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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleDecreased insulin secretion in islets from rats fed a low protein diet is associated with a reduced PKA alpha expressionpt_BR
dc.contributor.authorFerreira, Fpt_BR
dc.contributor.authorBarbosa, HCLpt_BR
dc.contributor.authorStoppiglia, LFpt_BR
dc.contributor.authorDelghingaro-Augusto, Vpt_BR
dc.contributor.authorPereira, EApt_BR
dc.contributor.authorBoschero, ACpt_BR
dc.contributor.authorCarneiro, EMpt_BR
unicamp.authorUniv Estadual Campinas, Inst Biol, Dept Fisiol & Biofis, Campinas, SP, Brazilpt_BR
dc.subjectlow protein dietpt_BR
dc.subjectinsulin secretionpt_BR
dc.subjectprotein kinase alphapt_BR
dc.subjectgene expressionpt_BR
dc.subject.wosPancreatic Beta-cellspt_BR
dc.subject.wosKinase-c Activationpt_BR
dc.subject.wosCalorie Malnutritionpt_BR
dc.subject.wosEnergy Malnutritionpt_BR
dc.subject.wosRegulatory Subunitspt_BR
dc.subject.wosFood Restrictionpt_BR
dc.subject.wosLimited Periodpt_BR
dc.subject.wosSertoli Cellspt_BR
dc.description.abstractA low protein diet has been shown to affect the amount and activity of several enzymes and to decrease insulin secretion by islets isolated from rats fed such a diet. To understand the mechanisms involved in this phenomenon, we investigated the effects of forskolin, a stimulator of adenylyl cyclase, on insulin secretion by pancreatic islets from rats fed a normal (17%; NP) or low (6%; LP) protein diet for 8 wk. Isolated islets were incubated for 1 h in Krebs-bicarbonate solution containing 8.3 mmol glucose/L, with or without 10 mumol forskolin/L. The forskolin-induced insulin secretion was higher in islets from NP rats than in those from LP rats (P < 0.05). Western blotting revealed that the amount of the alpha catalytic subunit of protein kinase A (PKAalpha) was 35% lower in islets from LP rats than in islets from NP rats (P < 0.05). Moreover, PKAalpha mRNA expression was reduced by 30% in islets from LP rats (P < 0.05). Our results indicated a possible relationship between a low protein diet and a reduction in PKAalpha expression. These alterations in PKAalpha may be responsible in part for the decreased insulin secretion by islets from rats fed a low protein
dc.relation.ispartofJournal Of Nutritionpt_BR
dc.relation.ispartofabbreviationJ. Nutr.pt_BR
dc.publisherAmer Inst Nutritionpt_BR
dc.identifier.citationJournal Of Nutrition. Amer Inst Nutrition, v. 134, n. 1, n. 63, n. 67, 2004.pt_BR
dc.sourceWeb of Sciencept_BR
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