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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleDecreased insulin secretion in islets from rats fed a low protein diet is associated with a reduced PKA alpha expressionpt_BR
dc.contributor.authorFerreira, Fpt_BR
dc.contributor.authorBarbosa, HCLpt_BR
dc.contributor.authorStoppiglia, LFpt_BR
dc.contributor.authorDelghingaro-Augusto, Vpt_BR
dc.contributor.authorPereira, EApt_BR
dc.contributor.authorBoschero, ACpt_BR
dc.contributor.authorCarneiro, EMpt_BR
unicamp.author.emailboschero@unicamp.brpt_BR
unicamp.authorUniv Estadual Campinas, Inst Biol, Dept Fisiol & Biofis, Campinas, SP, Brazilpt_BR
dc.subjectlow protein dietpt_BR
dc.subjectforskolinpt_BR
dc.subjectinsulin secretionpt_BR
dc.subjectprotein kinase alphapt_BR
dc.subjectgene expressionpt_BR
dc.subject.wosPancreatic Beta-cellspt_BR
dc.subject.wosKinase-c Activationpt_BR
dc.subject.wosCalorie Malnutritionpt_BR
dc.subject.wosEnergy Malnutritionpt_BR
dc.subject.wosRegulatory Subunitspt_BR
dc.subject.wosFood Restrictionpt_BR
dc.subject.wosGene-expressionpt_BR
dc.subject.wosLimited Periodpt_BR
dc.subject.wosSertoli Cellspt_BR
dc.subject.wosAdult-ratspt_BR
dc.description.abstractA low protein diet has been shown to affect the amount and activity of several enzymes and to decrease insulin secretion by islets isolated from rats fed such a diet. To understand the mechanisms involved in this phenomenon, we investigated the effects of forskolin, a stimulator of adenylyl cyclase, on insulin secretion by pancreatic islets from rats fed a normal (17%; NP) or low (6%; LP) protein diet for 8 wk. Isolated islets were incubated for 1 h in Krebs-bicarbonate solution containing 8.3 mmol glucose/L, with or without 10 mumol forskolin/L. The forskolin-induced insulin secretion was higher in islets from NP rats than in those from LP rats (P < 0.05). Western blotting revealed that the amount of the alpha catalytic subunit of protein kinase A (PKAalpha) was 35% lower in islets from LP rats than in islets from NP rats (P < 0.05). Moreover, PKAalpha mRNA expression was reduced by 30% in islets from LP rats (P < 0.05). Our results indicated a possible relationship between a low protein diet and a reduction in PKAalpha expression. These alterations in PKAalpha may be responsible in part for the decreased insulin secretion by islets from rats fed a low protein diet.pt
dc.relation.ispartofJournal Of Nutritionpt_BR
dc.relation.ispartofabbreviationJ. Nutr.pt_BR
dc.publisher.cityBethesdapt_BR
dc.publisher.countryEUApt_BR
dc.publisherAmer Inst Nutritionpt_BR
dc.date.issued2004pt_BR
dc.date.monthofcirculationJANpt_BR
dc.identifier.citationJournal Of Nutrition. Amer Inst Nutrition, v. 134, n. 1, n. 63, n. 67, 2004.pt_BR
dc.language.isoenpt_BR
dc.description.volume134pt_BR
dc.description.issuenumber1pt_BR
dc.description.firstpage63pt_BR
dc.description.lastpage67pt_BR
dc.rightsfechadopt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn0022-3166pt_BR
dc.identifier.wosidWOS:000188124900011pt_BR
dc.date.available2014-11-15T22:54:30Z
dc.date.available2015-11-26T17:22:25Z-
dc.date.accessioned2014-11-15T22:54:30Z
dc.date.accessioned2015-11-26T17:22:25Z-
dc.description.provenanceMade available in DSpace on 2014-11-15T22:54:30Z (GMT). No. of bitstreams: 1 WOS000188124900011.pdf: 266828 bytes, checksum: 6a20827f630a6ac075f232b0fdb8b45a (MD5) Previous issue date: 2004en
dc.description.provenanceMade available in DSpace on 2015-11-26T17:22:25Z (GMT). No. of bitstreams: 2 WOS000188124900011.pdf: 266828 bytes, checksum: 6a20827f630a6ac075f232b0fdb8b45a (MD5) WOS000188124900011.pdf.txt: 32547 bytes, checksum: f0bdb6bbb237e5ca2bcc67eb73664504 (MD5) Previous issue date: 2004en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/79351pt_BR
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/79351
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/79351-
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