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|Type:||Artigo de periódico|
|Title:||Decreased cholinergic stimulation of insulin secretion by islets from rats fed a low protein diet is associated with reduced protein kinase C alpha expression|
|Abstract:||Undernutrition has been shown to affect the autonomic nervous system, leading to permanent alterations in insulin secretion. To understand these interactions better, we investigated the effects of carbamyl-choline (CCh) and phorbol 12-myristate 13-acetate (PMA) on insulin secretion in pancreatic islets from rats fed a normal (17%; NP) or low (6%; LP) protein diet for 8 wk. Isolated islets were incubated for 1 h in Krebs-bicarbonate solution containing 8.3 mmol glucose/L, with or without PMA (400 nmoI/Q and CCh. Increasing concentrations of CCh (0.1 -1000 mumol/L) dose dependently increased insulin secretion by islets from both groups of rats. However, insulin secretion by islets from rats fed the NP diet was significantly higher than that of rats fed the LIP diet, and the dose-response curve to CCh was shifted to the right in islets from rats fed LIP with a 50% effective concentration (EC50) of 2.15 +/- 0.7 and 4.64 +/- 0.1 mumol CCh/L in islets of rats fed NP and LP diets, respectively (P < 0.05). PMA-induced insulin secretion was higher in islets of rats fed NP compared with those fed LIP. Western blotting revealed that the protein kinase (PK)Calpha and phospholipase (PL)Cbeta(1) contents of islets of rats fed LP were 30% lower than those of islets of rats fed NP (P < 0.05). In addition, PKCalpha mRNA expression was reduced by 50% in islets from rats fed LIP. In conclusion, a reduced expression of PKCa and PLCbeta(1), may be involved in the decreased insulin secretion by islets from LIP rats after stimulation with CCh and PMA. J. Nutr. 133: 695-699, 2003.|
|Subject:||low protein diet|
protein kinase C alpha
phospholipase C beta 1
|Editor:||Amer Inst Nutrition|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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