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Type: Artigo de periódico
Title: Decreased arginine and nitrite levels in nitrate reductase-deficient Arabidopsis thaliana plants impair nitric oxide synthesis and the hypersensitive response to Pseudomonas syringae
Author: Modolo, LV
Augusto, O
Almeida, IMG
Pinto-Maglio, CAF
Oliveira, HC
Seligman, K
Salgado, I
Abstract: Nitric oxide ((center dot)NO) produced in plants is implicated in defense responses against pathogens. (center dot)NO synthesis in such conditions has been attributed to a nitric oxide synthase (NOS)-like enzyme and, more recently, to a mitochondrial-dependent NO(2)(-)-reducing activity. In this work, we used an NR-deficient double mutant (nia1 nia2) of Arabidopsis thaliana that is deficient in endogenous NO(2)(-) to analyse the hypersensitive response (HR) against an avirulent strain of Pseudomonas syringae pv. maculicola (Psm). The inoculation of Psm into nia1 nia2 A. thaliana caused leaf chlorosis whereas the HR was induced in wild-type plants. (center dot)NO production in situ was substantially increased in wild-type but not in nia1 nia2 leaves following inoculation of Psm, as measured with the fluorescent (center dot)NO indicator 4,5-diaminofluorescein diacetate. However, the infiltration of L-arginine or NO(2)(-) into nia1 nia2 leaves triggered (center dot)NO production in situ. Moreover, co-infiltration of NO(2)(-) and Psm restored the HR in the leaves of nia1 nia2 plants. The total content of free amino acids, particularly L-arginine, was much lower in nia1 nia2 leaves compared to wild-type leaves. Overall, these results suggest that the HR is affected in NR-deficient plants because these plants lack L-arginine and NO(2)(-), two important endogenous substrates for (center dot)NO synthesis. (c) 2006 Elsevier Ireland Ltd. All rights reserved.
Subject: Arabidopsis thaliana
nitric oxide
nitrate reductase
hypersensitive response
Country: Irlanda
Editor: Elsevier Ireland Ltd
Citation: Plant Science. Elsevier Ireland Ltd, v. 171, n. 1, n. 34, n. 40, 2006.
Rights: fechado
Identifier DOI: 10.1016/j.plantsci.2006.02.010
Date Issue: 2006
Appears in Collections:Unicamp - Artigos e Outros Documentos

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