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|Type:||Artigo de periódico|
|Title:||Gingival crevicular fluid levels of MMP-8, MMP-9, TIMP-2, and MPO decrease after periodontal therapy|
|Author:||MARCACCINI, Andrea M.|
MESCHIARI, Cesar A.
ZUARDI, Leonardo R.
SOUSA, Tiago Sampaio de
TABA JR., Mario
TEOFILO, Juliana M.
JACOB-FERREIRA, Anna L. B.
TANUS-SANTOS, Jose E.
NOVAES JR., Arthur B.
GERLACH, Raquel F.
|Abstract:||P>Background This study aimed at comparing the levels of matrix metalloproteinase (MMP)-8, tissue Inhibitor of MMPs (TIMP)-1 and TIMP-2, Myeloperoxidase (MPO), and MMP-9 in the gingival crevicular fluid (GCF) of chronic periodontitis (CP) patients and controls at baseline and 3 months after non-surgical therapy. Materials and Methods GCF was collected from one site of 15 control subjects and 27 CP patients. MMP-8, MMP-9, TIMP-1, and TIMP-2 were determined by Enzyme-linked immunoabsorbent assay; different forms of MMP-9, by gelatin zymography; and MPO, colorimetrically. Results At baseline, higher levels of MMP-8, TIMP-2, MPO, and the 87 kDa-MMP-9 were found in patients compared with controls (p < 0.001), and these molecules decreased after therapy (p < 0.03). There were no differences between the groups with respect to the higher molecular forms of MMP-9 (180, 130, 92 kDa) or total MMP-9 at baseline. No differences were observed in TIMP-1 levels. In controls, decreased levels of TIMP-2 and the higher molecular forms of MMP-9 (180, 130, 92 kDa) were found 3 months after therapy compared with baseline (p < 0.01). Conclusions Higher levels of MMP-8, TIMP-2, MPO, and 87 kDa MMP-9 were found in the GCF of patients compared with controls, and these markers decreased 3 months after periodontal therapy.|
gingival crevicular fluid
|Editor:||WILEY-BLACKWELL PUBLISHING, INC|
|Appears in Collections:||FCM - Artigos e Outros Documentos|
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